Human Transcription factor SOX-2 ELISA Kit | SOX2 elisa kit

Human Transcription factor SOX-2 ELISA Kit

Cat. Num. AAA764706

• Gene Names: SOX2; ANOP3; MCOPS3
• Reactivity: Human
• Synonyms: Transcription factor SOX-2; Human Transcription factor SOX-2 ELISA Kit; SOX2 (Sex Determining Region Y Box Protein 2)/ANOP3/MCOPS3/SRY (sex determining region Y)-box 2/SRY-related HMG-box gene 2/transcription factor SOX2/transcription factor SOX-2; SOX2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SOST. No significant cross-reactivity or interference between SOST and analogues was observed.
• Sequence Length: 317

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: <18.75pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SOST were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SOST were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOX2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-SOST antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-SOST antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SOST amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SOST can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 28195386
• NCBI GeneID: 6657
• NCBI Accession #: NP_003097.1
• NCBI GenBank Nucleotide #: NM_003106.3
• Molecular Weight: 34,310 Da
• NCBI Official Full Name: transcription factor SOX-2
• NCBI Official Synonym Full Names: SRY-box 2
• NCBI Official Symbol: SOX2
• NCBI Official Synonym Symbols: ANOP3; MCOPS3
• NCBI Protein Information: transcription factor SOX-2
• UniProt Protein Name: Transcription factor SOX-2
• Protein Family: Transcription factor
• UniProt Gene Name: SOX2
• UniProt Entry Name: SOX2_HUMAN

NCBI Description

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]

Uniprot Description

SOX2: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.

Protein type: DNA-binding; Transcription factor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 3q26.3-q27

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin DNA binding; DNA binding; miRNA binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: adenohypophysis development; alveolus development; cell cycle arrest; cerebral cortex development; detection of mechanical stimulus involved in equilibrioception; detection of mechanical stimulus involved in sensory perception of sound; diencephalon morphogenesis; endodermal cell fate specification; establishment and/or maintenance of chromatin architecture; eye development; forebrain development; forebrain neuron differentiation; glial cell fate commitment; inner ear development; inner ear morphogenesis; male genitalia development; negative regulation of epithelial cell proliferation; negative regulation of neuron differentiation; negative regulation of osteoblast differentiation; negative regulation of transcription from RNA polymerase II promoter; neuron fate commitment; olfactory placode formation; osteoblast differentiation; pigment biosynthetic process; pituitary gland development; positive regulation of cell-cell adhesion; positive regulation of epithelial cell differentiation; positive regulation of MAPKKK cascade; positive regulation of neuroblast proliferation; positive regulation of neuron differentiation; positive regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of caspase activity; regulation of gene expression; regulation of transcription, DNA-dependent; response to retinoic acid; response to wounding; retina morphogenesis in camera-type eye; somatic stem cell maintenance; stem cell differentiation; tissue regeneration; tongue development; transcription from RNA polymerase II promoter

Disease: Microphthalmia, Syndromic 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia