Mouse sortilin 1 ELISA Kit | SORT1 elisa kit

Mouse Sortilin, SORT1 ELISA Kit

Cat. Num. AAA939726

• Gene Names: Sort1; Ntr3; Ntsr3; AI852375; 2900053A11Rik
• Reactivity: Mouse
• Synonyms: sortilin 1; Mouse Sortilin; SORT1 ELISA Kit; Mouse Sortilin (SORT1) ELISA kit; Gp95; NT3; neurotensin receptor 3; SORT1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse SORT1. No significant cross-reactivity or interference between mouse SORT1 and analogues was observed.
• Sequence Length: 825

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 6.25 pg/ml-400 pg/ml
• Sensitivity: 1.56 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SORT1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SORT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SORT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SORT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SORT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 409264681
• NCBI GeneID: 20661
• NCBI Accession #: NP_001258528.1
• NCBI GenBank Nucleotide #: NM_001271599.1
• UniProt Accession #: Q6PHU5; Q3UHE2; Q8K043; Q9QXW6; A2AEE8
• Molecular Weight: 91,200 Da
• NCBI Official Full Name: sortilin isoform 1
• NCBI Official Synonym Full Names: sortilin 1
• NCBI Official Symbol: Sort1
• NCBI Official Synonym Symbols: Ntr3; Ntsr3; AI852375; 2900053A11Rik
• NCBI Protein Information: sortilin; mNTR3; neurotensin receptor 3
• UniProt Protein Name: Sortilin
• Protein Family: Sortilin
• UniProt Gene Name: Sort1
• UniProt Synonym Gene Names: NTR3; mNTR3
• UniProt Entry Name: SORT_MOUSE

Uniprot Description

SORT1: Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. A common polymorphism located in a non-coding region between CELSR2 and PSRC1 alters a CEBP transcription factor binding site and is responsible for changes in hepatic expression of SORT1. Altered SORT1 expression in liver affects low density lipoprotein cholesterol levels in plasma and is associated with susceptibility to myocardial infarction. Belongs to the VPS10-related sortilin family. SORT1 subfamily.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: Golgi apparatus; cell surface; intracellular membrane-bound organelle; clathrin-coated vesicle; endoplasmic reticulum; lysosome; early endosome; dendrite; cytoplasmic membrane-bound vesicle; integral to membrane; coated pit; trans-Golgi network transport vesicle; membrane; cell soma; perinuclear region of cytoplasm; plasma membrane; nucleus; endosome

Molecular Function: protein binding; enzyme binding; nerve growth factor receptor activity; nerve growth factor binding; neurotensin receptor activity, non-G-protein coupled

Biological Process: ossification; nerve growth factor receptor signaling pathway; endosome transport via multivesicular body sorting pathway; multicellular organismal development; vesicle organization and biogenesis; Golgi to endosome transport; myotube differentiation; endocytosis; response to insulin stimulus; G-protein coupled receptor protein signaling pathway; induction of apoptosis via death domain receptors; neuropeptide signaling pathway; regulation of gene expression; transport; glucose import; endosome to lysosome transport; plasma membrane to endosome transport; negative regulation of lipoprotein lipase activity; cell differentiation