RSV (B1) glycoprotein G/RSV-G Protein Recombinant Protein | RSV-G recombinant protein

Recombinant Human RSV (B1) glycoprotein G/RSV-G Protein (His Tag)

Cat. Num. AAA2569564

• Purity: >95% as determined by SDS-PAGE
• Synonyms: RSV (B1) glycoprotein G/RSV-G Protein; Recombinant Human RSV (B1) glycoprotein G/RSV-G Protein (His Tag); G Protein; RSV; RSV-G recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
• Sequence: His 67-Ala 299

• Species: Respiratory Syncytial Virus (RSV)
• Tag: C-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Protein Construction: A DNA sequence encoding the extracellular domain of human RSV strain B1 glycoprotein G (O36633-1)(His 67-Ala 299) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for RSV-G recombinant protein

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. HRSV G protein is a type II glycoprotein of 289-299 amino acids (depending on the virus strain) with a signal/anchor hydrophobic domain and is extensively modified by the addition of both N-and O-linked oligosaccharides to achieve the mature form of 8-9kDa. The C-terminal ectodomain of the G protein has a central region and four cysteines which are conserved in all HRSV isolates and have been proposed as the putative receptor binding site. The G protein mediates attachment of the virus to the host cell membrane by interacting with heparan sulfate, initiating the infection. As similar to mucins in amino acid compositions, the RSV G protein can interact with host CX3CR1, the receptor for the CX3C chemokine fractalkine, and thus modulates the immune response and facilitate infection. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors. Unlike the other paramyxovirus attachment proteins, HRSV-G lacks both neuraminidase and hemagglutinating activities.

Product Categories/Family for RSV-G recombinant protein

Virus related Protein

NCBI and Uniprot Product Information

• NCBI GI #: 9629205
• NCBI GeneID: 1489824
• NCBI Accession #: NP_056862.1
• NCBI GenBank Nucleotide #: NC_001781.1
• UniProt Accession #: O36633
• Molecular Weight: 32,967 Da
• NCBI Official Full Name: attachment glycoprotein
• NCBI Official Symbol: G
• NCBI Protein Information: G; attachment glycoprotein
• UniProt Protein Name: Major surface glycoprotein G
• UniProt Gene Name: G
• UniProt Synonym Gene Names: mG
• UniProt Entry Name: GLYC_HRSVB

Uniprot Description

Function: Attaches the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. Interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, lacks both neuraminidase and hemagglutinating activities

By similarity.Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors

By similarity.

Subunit structure: Homooligomer. Interacts (via N-terminus) with protein M. Interacts with protein F; this interaction occurs on the surface of infected cells. Interacts with protein SH. Interacts with host CX3CR1; this interaction modulates host immune response

By similarity.

Subcellular location: Virion membrane. Host cell surface

By similarity. Isoform Secreted glycoprotein G: Secreted.

Domain: Contains a linear heparin binding domain essential for virus attachment to the host.

Post-translational modification: May carry 40-80 separate O-linked carbohydrate chains distributed among the 91 serine and threonine residues.Palmitoylated.

Sequence similarities: Belongs to the pneumoviruses glycoprotein G family.