Sorting Nexin 13 Recombinant Protein | SNX13 recombinant protein

Recombinant Sorting Nexin 13 (SNX13)

Cat. Num. AAA2031295

• Gene Names: SNX13; RGS-PX1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Sorting Nexin 13; Recombinant Sorting Nexin 13 (SNX13); SNX13 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; ANII DEPLQQVIQF SLRDYVQYWY YTLSDDESFL LEIRQTLQNA LIQFATRSKE IDWQPYFTTR IVDDFGTHLR VFRKAQQKIT EKDDQVKGTA EDLVDTFFEV EVEMEKEVCR DLVCTSPKDE EGFLRDLCEV LLYLLLPPGD FQNKIMRYFV REILARGILL PLINQLSDPDYINQYVIWMI RDSN
• Sequence Length: 957

• Applicable Applications for SNX13 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 26.1kDa
• Accurate Molecular Mass (KD): 29kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ala97~Asn284
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 17864088
• NCBI GeneID: 23161
• NCBI Accession #: NP_055947.1
• NCBI GenBank Nucleotide #: NM_015132.4
• Molecular Weight: 110,960 Da
• NCBI Official Full Name: sorting nexin-13
• NCBI Official Synonym Full Names: sorting nexin 13
• NCBI Official Symbol: SNX13
• NCBI Official Synonym Symbols: RGS-PX1
• NCBI Protein Information: sorting nexin-13
• UniProt Protein Name: Sorting nexin-13
• Protein Family: Sorting nexin
• UniProt Gene Name: SNX13
• UniProt Synonym Gene Names: KIAA0713
• UniProt Entry Name: SNX13_HUMAN

NCBI Description

This gene encodes a PHOX domain- and RGS domain-containing protein that belongs to the sorting nexin (SNX) family and the regulator of G protein signaling (RGS) family. The PHOX domain is a phosphoinositide binding domain, and the SNX family members are involved in intracellular trafficking. The RGS family members are regulatory molecules that act as GTPase activating proteins for G alpha subunits of heterotrimeric G proteins. The RGS domain of this protein interacts with G alpha(s), accelerates its GTP hydrolysis, and attenuates G alpha(s)-mediated signaling. Overexpression of this protein delayes lysosomal degradation of the epidermal growth factor receptor. Because of its bifunctional role, this protein may link heterotrimeric G protein signaling and vesicular trafficking. [provided by RefSeq, Jul 2008]

Uniprot Description

SNX13: May be involved in several stages of intracellular trafficking. May play a role in endosome homeostasis. Acts as a GAP for Galphas. Belongs to the sorting nexin family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: early endosome membrane; early endosome

Molecular Function: phosphoinositide binding; phosphatidylinositol 3-phosphate binding

Biological Process: intracellular protein transport; positive regulation of GTPase activity