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Small Nuclear Ribonucleoprotein Recombinant Protein | SNRPD3 recombinant protein

Recombinant Human Small Nuclear Ribonucleoprotein Polypeptide D3, Sf9

Gene Names
SNRPD3; SMD3; Sm-D3
Applications
Western Blot
Purity
Greater than 80% as determined by SDS-PAGE.
Synonyms
Small Nuclear Ribonucleoprotein; Recombinant Human Small Nuclear Ribonucleoprotein Polypeptide D3; Sf9; SNRPD3 Human; Small Nuclear Ribonucleoprotein Polypeptide D3 Human Recombinant; Small nuclear ribonucleoprotein D3 polypeptide 18kDa; Sm-D3; snRNP core protein D3; SNRPD3; SNRPD3 recombinant protein
Ordering
For Research Use Only!
Host
Sf9 Insect Cells
Purity/Purification
Greater than 80% as determined by SDS-PAGE.
Form/Format
SNRPD3 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Sterile Filtered clear solution.
Concentration
0.4-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. (varies by lot)
Sequence Length
126
Applicable Applications for SNRPD3 recombinant protein
Western Blot (WB)
Application Notes
Western blot with anti-Sm positive patient sera.
Immunological Functions
1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.
Related Product Information for SNRPD3 recombinant protein
Description: SNRPD3 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 14,739 Dalton. SNRPD3 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.

Introduction: SNRPD3 is a small nuclear ribonucleoprotein (snRNPs) that contains the spliceosome in eukaryotes. SNRPD3 is essential for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Alternative splicing happens in this locus and two transcript variants encoding the same protein were branded.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,292 Da
NCBI Official Full Name
small nuclear ribonucleoprotein Sm D3
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein D3 polypeptide 18kDa
NCBI Official Symbol
SNRPD3
NCBI Official Synonym Symbols
SMD3; Sm-D3
NCBI Protein Information
small nuclear ribonucleoprotein Sm D3; snRNP core protein D3
UniProt Protein Name
Small nuclear ribonucleoprotein Sm D3
UniProt Gene Name
SNRPD3
UniProt Synonym Gene Names
Sm-D3
UniProt Entry Name
SMD3_HUMAN

NCBI Description

This gene encodes a core component of the spliceosome, which is a nuclear ribonucleoprotein complex that functions in pre-mRNA splicing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

snRNP D3: Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner. Belongs to the snRNP core protein family.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: small nuclear ribonucleoprotein complex; nucleoplasm; spliceosome; cytoplasm; snRNP U1; cytosol; U12-dependent spliceosome

Molecular Function: protein binding; enzyme binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; RNA splicing; histone mRNA metabolic process; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription

Research Articles on SNRPD3

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Product Notes

The SNRPD3 snrpd3 (Catalog #AAA145043) is a Recombinant Protein produced from Sf9 Insect Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Small Nuclear Ribonucleoprotein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blot with anti-Sm positive patient sera. Researchers should empirically determine the suitability of the SNRPD3 snrpd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Small Nuclear Ribonucleoprotein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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