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Western Blot (WB) (Western blot analysis of SNRPD3 Antibody (C-term) in MCF-7, Jurkat cell line lysates (35ug/lane). SNRPD3 (arrow) was detected using the purified Pab.)

Rabbit anti-Human SNRPD3 Polyclonal Antibody | anti-SNRPD3 antibody

SNRPD3, CT (SNRPD3, Small nuclear ribonucleoprotein Sm D3, snRNP core protein D3) (AP)

Gene Names
SNRPD3; SMD3; Sm-D3
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SNRPD3; Polyclonal Antibody; CT (SNRPD3; Small nuclear ribonucleoprotein Sm D3; snRNP core protein D3) (AP); snRNP core protein D3; anti-SNRPD3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-SNRPD3 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SNRPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~126 amino acids from the C-terminal region of human SNRPD3.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SNRPD3 Antibody (C-term) in MCF-7, Jurkat cell line lysates (35ug/lane). SNRPD3 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of SNRPD3 Antibody (C-term) in MCF-7, Jurkat cell line lysates (35ug/lane). SNRPD3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(SNRPD3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SNRPD3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (SNRPD3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SNRPD3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SNRPD3 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (SNRPD3 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-SNRPD3 antibody
The protein belongs to the small nuclear ribonucleoprotein core protein family. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis.
Product Categories/Family for anti-SNRPD3 antibody
References
Gonsalvez, G.B., et.al., J. Cell Biol. 178 (5), 733-740 (2007). Ma, Y., et.al., Structure 13 (6), 883-892 (2005). Mahler, M., et.al., Arthritis Res. Ther. 7 (1), R19-R29 (2005).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,292 Da
NCBI Official Full Name
small nuclear ribonucleoprotein Sm D3
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein D3 polypeptide 18kDa
NCBI Official Symbol
SNRPD3
NCBI Official Synonym Symbols
SMD3; Sm-D3
NCBI Protein Information
small nuclear ribonucleoprotein Sm D3; snRNP core protein D3
UniProt Protein Name
Small nuclear ribonucleoprotein Sm D3
UniProt Gene Name
SNRPD3
UniProt Synonym Gene Names
Sm-D3
UniProt Entry Name
SMD3_HUMAN

NCBI Description

This gene encodes a core component of the spliceosome, which is a nuclear ribonucleoprotein complex that functions in pre-mRNA splicing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

snRNP D3: Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner. Belongs to the snRNP core protein family.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: small nuclear ribonucleoprotein complex; nucleoplasm; spliceosome; cytoplasm; snRNP U1; cytosol; U12-dependent spliceosome

Molecular Function: protein binding; enzyme binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; RNA splicing; histone mRNA metabolic process; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription

Research Articles on SNRPD3

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Product Notes

The SNRPD3 snrpd3 (Catalog #AAA6349419) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SNRPD3, CT (SNRPD3, Small nuclear ribonucleoprotein Sm D3, snRNP core protein D3) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SNRPD3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SNRPD3 snrpd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNRPD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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