Synaptosomal Associated Protein 23kDa Recombinant Protein | SNAP23 recombinant protein

Recombinant Synaptosomal Associated Protein 23kDa (SNAP23)

Cat. Num. AAA2033117

• Gene Names: Snap23; Sndt; 23kDa; Syndet; SNAP-23; AA408749
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Synaptosomal Associated Protein 23kDa; Recombinant Synaptosomal Associated Protein 23kDa (SNAP23); SNAP23A; SNAP23B; Vesicle-membrane fusion protein SNAP-23; SNAP23 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MDNLSPEEVQ LRAHQVTDES LESTRRILGL AIESQDAGIK TITMLDEQGEQLNRIEEGMD QINKDMREAE KTLTELNKCC GLCICPCNRT KNFESGKNYKATWGDGGDNS PSNVVSKQPS RITNGQPQQT TGAASGGYIK RITNDAREDEMEENLTQVGS ILGNLKNMAL DMGNEIDAQN QQIQKITEKA DTNKNRIDIANTRAKKLIDS
• Sequence Length: 210

• Applicable Applications for SNAP23 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 27.1kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Ser210
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 295317330
• NCBI GeneID: 20619
• NCBI Accession #: NP_001171264.1
• NCBI GenBank Nucleotide #: NM_001177793.1
• Molecular Weight: 23,261 Da
• NCBI Official Full Name: synaptosomal-associated protein 23 isoform b
• NCBI Official Synonym Full Names: synaptosomal-associated protein 23
• NCBI Official Symbol: Snap23
• NCBI Official Synonym Symbols: Sndt; 23kDa; Syndet; SNAP-23; AA408749
• NCBI Protein Information: synaptosomal-associated protein 23
• UniProt Protein Name: Synaptosomal-associated protein 23
• Protein Family: Synaptosomal-associated protein
• UniProt Gene Name: Snap23
• UniProt Synonym Gene Names: Sndt; SNAP-23
• UniProt Entry Name: SNP23_MOUSE

Uniprot Description

SNAP23: a member of the SNAP-25 family of SNARE proteins. Structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternatively spliced isoforms have been described.

Protein type: Vesicle

Cellular Component: azurophil granule; cell junction; cytoplasm; cytoplasmic vesicle; focal adhesion; mast cell granule; membrane; neuron projection; nucleoplasm; plasma membrane; SNARE complex; specific granule; synapse; terminal button

Molecular Function: protein binding; SNAP receptor activity; syntaxin binding

Biological Process: exocytosis; histamine secretion by mast cell; protein complex assembly; protein transport; regulation of exocytosis; synaptic vesicle fusion to presynaptic membrane; synaptic vesicle priming; transport; vesicle-mediated transport