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Western Blot (WB) (Western Blot: Sample: Lane1: Mouse Serum; Lane2: Mouse Placenta Tissue; Lane3: Mouse Lung Tissue; Lane4: Mouse Hela Cells.)

Rabbit anti-Mouse Synaptosomal Associated Protein 23kDa Polyclonal Antibody | anti-SNAP23 antibody

Synaptosomal Associated Protein 23kDa (SNAP23) Polyclonal Antibody

Gene Names
Snap23; Sndt; 23kDa; Syndet; SNAP-23; AA408749
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Synaptosomal Associated Protein 23kDa; Polyclonal Antibody; Synaptosomal Associated Protein 23kDa (SNAP23) Polyclonal Antibody; SNAP23A; SNAP23B; Vesicle-membrane fusion protein SNAP-23; anti-SNAP23 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SNAP23. It has been selected for its ability to recognize SNAP23 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
210
Applicable Applications for anti-SNAP23 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human, Mouse
Organism Species
Mouse
Fragment
SNAP23 (Met1~Ser210)
Quality Control
Content: The quality control contains recombinant SNAP23 (Met1~Ser210) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2068626
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Serum; Lane2: Mouse Placenta Tissue; Lane3: Mouse Lung Tissue; Lane4: Mouse Hela Cells.)

Western Blot (WB) (Western Blot: Sample: Lane1: Mouse Serum; Lane2: Mouse Placenta Tissue; Lane3: Mouse Lung Tissue; Lane4: Mouse Hela Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant SNAP23, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant SNAP23, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Liver Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Stomach Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Stomach Tissue)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,261 Da
NCBI Official Full Name
synaptosomal-associated protein 23 isoform a
NCBI Official Synonym Full Names
synaptosomal-associated protein 23
NCBI Official Symbol
Snap23
NCBI Official Synonym Symbols
Sndt; 23kDa; Syndet; SNAP-23; AA408749
NCBI Protein Information
synaptosomal-associated protein 23
UniProt Protein Name
Synaptosomal-associated protein 23
UniProt Gene Name
Snap23
UniProt Synonym Gene Names
Sndt; SNAP-23

Uniprot Description

SNAP23: a member of the SNAP-25 family of SNARE proteins. Structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternatively spliced isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 2 60.37 cM|2 E5

Cellular Component: azurophil granule; cell; cell junction; cell-cell adherens junction; cytoplasm; cytoplasmic vesicle; extracellular exosome; focal adhesion; intracellular; membrane; neuron projection; nucleoplasm; plasma membrane; SNARE complex; specific granule; synapse; terminal bouton

Molecular Function: protein binding; SNAP receptor activity; syntaxin binding

Biological Process: exocytic insertion of neurotransmitter receptor to postsynaptic membrane; exocytosis; histamine secretion by mast cell; protein complex assembly; protein transport; regulation of exocytosis; synaptic vesicle fusion to presynaptic membrane; synaptic vesicle priming; transport; vesicle-mediated transport

Research Articles on SNAP23

Similar Products

Product Notes

The SNAP23 snap23 (Catalog #AAA2032712) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Synaptosomal Associated Protein 23kDa (SNAP23) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Synaptosomal Associated Protein 23kDa can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SNAP23 snap23 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Synaptosomal Associated Protein 23kDa, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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