Human structural maintenance of chromosomes 3 ELISA Kit | SMC3 elisa kit

Human Structural maintenance of chromosomes protein 3, SMC3 ELISA Kit

Cat. Num. AAA9329719

• Gene Names: SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
• Reactivity: Human
• Synonyms: structural maintenance of chromosomes 3; Human Structural maintenance of chromosomes protein 3; SMC3 ELISA Kit; Human Structural maintenance of chromosomes protein 3 (SMC3) ELISA kit; BAM; BMH; CDLS3; CSPG6; HCAP; SMC3L1; basement membrane-associated chondroitin proteoglycan; chondroitin sulfate proteoglycan 6 (bamacan) ; chromosome-associated polypeptide; SMC3 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human SMC3 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml - 1000 pg/ml.
• Sensitivity: 5.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated SMC3 concentrations in Human serum, plasma and other body fluids. Using Purified Human SMC3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SMC3 and SMC3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SMC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for SMC3 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated SMC3 concentrations in Human serum, plasma and other body fluids. Using Purified Human SMC3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SMC3 and SMC3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SMC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885399
• NCBI GeneID: 9126
• NCBI Accession #: NP_005436.1
• NCBI GenBank Nucleotide #: NM_005445.3
• UniProt Accession #: Q9UQE7; O60464; Q5T482; A8K156
• Molecular Weight: 141,542 Da
• NCBI Official Full Name: structural maintenance of chromosomes protein 3
• NCBI Official Synonym Full Names: structural maintenance of chromosomes 3
• NCBI Official Symbol: SMC3
• NCBI Official Synonym Symbols: BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
• NCBI Protein Information: structural maintenance of chromosomes protein 3; SMC protein 3; chromosome-associated polypeptide; chondroitin sulfate proteoglycan 6 (bamacan); basement membrane-associated chondroitin proteoglycan
• UniProt Protein Name: Structural maintenance of chromosomes protein 3
• UniProt Gene Name: SMC3
• UniProt Synonym Gene Names: BAM; BMH; CSPG6; SMC3L1; SMC protein 3; SMC-3; Bamacan; hCAP
• UniProt Entry Name: SMC3_HUMAN

NCBI Description

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SMC3: Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. Interacts with MXI1, MXD3 and MXD4. Interacts with SYCP2. Found in a complex with SMC1A, CDCA5 and RAD21, PDS5A/APRIN and PDS5B/SCC-112. Forms a heterodimer with SMC1A or SMC1B in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Also found in meiosis-specific cohesin complexes. Interacts with NUMA1, and forms a ternary complex with KIF3B and KIFAP3, suggesting a function in tethering the chromosomes to the spindle pole and in chromosome movement. Interacts with PDS5A and WAPAL; regulated by SMC3 acetylation. Belongs to the SMC family. SMC3 subfamily.

Protein type: DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: cohesin complex; nucleoplasm; spindle pole; lateral element; cohesin core heterodimer; nuclear matrix; cytoplasm; basement membrane; chromosome; nucleus; chromatin; cytosol; chromosome, pericentric region

Molecular Function: dynein binding; protein binding; protein heterodimerization activity; microtubule motor activity; chromatin binding; ATP binding

Biological Process: mitosis; meiosis; mitotic spindle organization and biogenesis; cell division; stem cell maintenance; mitotic sister chromatid cohesion; sister chromatid cohesion; negative regulation of DNA endoreduplication; mitotic cell cycle; DNA repair; signal transduction; regulation of DNA replication

Disease: Cornelia De Lange Syndrome 3