Slit Homolog 2 (Slit2) Recombinant Protein | Slit2 recombinant protein

Recombinant Slit Homolog 2 (Slit2)

Cat. Num. AAA2011861

• Gene Names: Slit2; Slil3; Drad-1; mKIAA4141; E030015M03Rik; E130320P19Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Slit Homolog 2 (Slit2); Recombinant Slit Homolog 2 (Slit2); Slit2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-QCSCSGSTVD CHGLALRSVP RNIPRNTERL DLNGNNITRI TKIDFAGLRH LRVLQLMENR ISTIERGAFQ DLKELERLRL NRNNLQLFPE LLFLGTAKLY RLDLSENQIQ AIPRKAFRGA VDIKNLQLDY NQISCIEDGA FRALRDLEVL TLNNNNITRL SVASFNH
• Sequence Length: 1521

• Applicable Applications for Slit2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gln31~His197 (Accession # Q9R1B9) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 166064058
• NCBI GeneID: 20563
• NCBI Accession #: NP_848919.3
• NCBI GenBank Nucleotide #: NM_178804.3
• UniProt Accession #: Q9R1B9; Q9Z166; E9QKB4
• Molecular Weight: 20.6kDa
• NCBI Official Full Name: slit homolog 2 protein
• NCBI Official Synonym Full Names: slit homolog 2 (Drosophila)
• NCBI Official Symbol: Slit2
• NCBI Official Synonym Symbols: Slil3; Drad-1; mKIAA4141; E030015M03Rik; E130320P19Rik
• NCBI Protein Information: slit homolog 2 protein; slit-2; neurogenic extracellular slit protein; downregulated during adipocyte differentiation-1
• UniProt Protein Name: Slit homolog 2 protein
• Protein Family: Slit homolog 2 protein
• UniProt Gene Name: Slit2
• UniProt Synonym Gene Names: Slit-2
• UniProt Entry Name: SLIT2_MOUSE

NCBI Description

The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

SLIT2: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth- stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post- crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; plasma membrane

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; laminin-1 binding; identical protein binding; proteoglycan binding; protein binding; protein homodimerization activity; Roundabout binding; chemorepellent activity; calcium ion binding; GTPase inhibitor activity; receptor binding

Biological Process: negative regulation of small GTPase mediated signal transduction; axon guidance; positive regulation of apoptosis; multicellular organismal development; negative chemotaxis; motor axon guidance; negative regulation of smooth muscle cell migration; negative regulation of axon extension; chemotaxis; corticospinal neuron axon guidance through the spinal cord; induction of negative chemotaxis; negative regulation of cell proliferation; cell-cell adhesion; negative regulation of protein amino acid phosphorylation; ureteric bud development; negative regulation of leukocyte chemotaxis; cell differentiation; negative regulation of cell migration; nervous system development; negative regulation of actin filament polymerization; in utero embryonic development; olfactory bulb development; chemorepulsion involved in postnatal olfactory bulb interneuron migration; dorsal/ventral axon guidance; axon extension involved in axon guidance; branching morphogenesis of a tube; axonogenesis; cell migration during sprouting angiogenesis; negative regulation of inflammatory response; negative regulation of catalytic activity; negative regulation of vascular permeability; negative regulation of cell growth; metanephros development; neurite morphogenesis; retinal ganglion cell axon guidance; telencephalon cell migration