Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Slit Homolog 2 Polyclonal Antibody | anti-Slit2 antibody
Polyclonal Antibody to Slit Homolog 2 (Slit2)
MGHHHHHHSG SEF- CQM IKCKHGKCRL SGVGQPYCEC NSGFTGDSCD REISCRGERI RDYYQKQQGY AACQTTKKVS RLECRGGCAG GQCCGPLRSK RRKYSFECTD
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
SLIT2: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth- stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post- crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5|5 B3
Cellular Component: cytoplasm; extracellular region; extracellular space; plasma membrane
Molecular Function: calcium ion binding; chemorepellent activity; GTPase inhibitor activity; heparan sulfate proteoglycan binding; heparin binding; identical protein binding; laminin-1 binding; protein binding; protein homodimerization activity; proteoglycan binding; receptor binding; Roundabout binding
Biological Process: axon extension involved in axon guidance; axon guidance; axonogenesis; branching morphogenesis of a tube; cell differentiation; cell migration during sprouting angiogenesis; central nervous system projection neuron axonogenesis; chemorepulsion involved in postnatal olfactory bulb interneuron migration; chemotaxis; corticospinal neuron axon guidance through spinal cord; dorsal/ventral axon guidance; in utero embryonic development; induction of negative chemotaxis; metanephros development; motor axon guidance; multicellular organism development; negative chemotaxis; negative regulation of actin filament polymerization; negative regulation of axon extension; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of inflammatory response; negative regulation of leukocyte chemotaxis; negative regulation of protein phosphorylation; negative regulation of small GTPase mediated signal transduction; negative regulation of smooth muscle cell migration; negative regulation of vascular permeability; nervous system development; neuron projection morphogenesis; olfactory bulb development; positive regulation of apoptosis; retinal ganglion cell axon guidance; telencephalon cell migration; ureteric bud development
Research Articles on Slit2
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Product Notes
The Slit2 slit2 (Catalog #AAA2028504) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Slit Homolog 2 (Slit2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Slit Homolog 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the Slit2 slit2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- CQM IKCKHGKCRL SGVGQPYCEC NSGFTGDSCD REISCRGERI RDYYQKQQGY AACQTTKKVS RLECRGGCAG GQCCGPLRSK RRKYSFECTD. It is sometimes possible for the material contained within the vial of "Slit Homolog 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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