Typical Testing Data/Standard Curve (for reference only)
Mouse Sodium/calcium exchanger 1 ELISA Kit | Slc8a1 elisa kit
Mouse Sodium/calcium exchanger 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/calcium exchanger 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forSodium/calcium exchanger 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Sodium/calcium exchanger 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Sodium/calcium exchanger 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
NCX1: Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores. Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass
Cellular Component: basolateral plasma membrane; cell projection; cytosol; dendritic shaft; dendritic spine; integral to membrane; integral to plasma membrane; membrane; microtubule; mitochondrion; plasma membrane; sarcolemma; T-tubule; Z disc
Molecular Function: ankyrin binding; antiporter activity; calcium:sodium antiporter activity; calmodulin binding; cytoskeletal protein binding; metal ion binding; protein binding
Biological Process: calcium ion homeostasis; calcium ion transport; cardiac muscle cell development; cardiac muscle contraction; cell communication; cellular calcium ion homeostasis; cellular sodium ion homeostasis; elevation of cytosolic calcium ion concentration; embryonic heart tube development; embryonic placenta development; heart morphogenesis; ion transport; muscle fiber development; positive regulation of bone mineralization; post-embryonic development; reduction of cytosolic calcium ion concentration; regulation of calcium ion transport; regulation of heart rate; regulation of sodium ion transport; regulation of the force of heart contraction; response to ATP; response to hypoxia; sodium ion transport; transmembrane transport; transport; vascular smooth muscle contraction
Research Articles on Slc8a1
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Product Notes
The Mouse Slc8a1 slc8a1 (Catalog #AAA2883122) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2883122 ELISA Kit recognizes Mouse Slc8a1. It is sometimes possible for the material contained within the vial of "Sodium/calcium exchanger 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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