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Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A172 cells, Raji cells, Primary antibody: 122335(FAIM2 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Rabbit anti-Human FAIM2/LFG Polyclonal Antibody | anti-FAIM2/LFG antibody

FAIM2/LFG Rabbit pAb

Gene Names
FAIM2; LFG; LFG2; NGP35; NMP35; TMBIM2
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
FAIM2/LFG; Polyclonal Antibody; FAIM2/LFG Rabbit pAb; LFG; LFG2; NGP35; NMP35; TMBIM2; anti-FAIM2/LFG antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-FAIM2/LFG antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
Immunogen
Fusion protein of human FAIM2
Conjugation
Unconjugated
Molecular Function
FAIM2 (Fas apoptotic inhibitory molecule 2), also known as LFG (Protein lifeguard), TMBIM2 (Transmembrane BAX inhibitor motif-containing protein 2) and NMP35 (neural membrane protein 35), is a 316 amino acid multipass membrane protein that uniquely protects cells from Fas-induced apoptosis. Though widely expressed, FAIM2 expression is highest in hippocampus. FAIM2 contains seven transmembrane domains and resembles Bax Inhibitor-1, another anti-apoptotic protein.
Modification
Unmodified
Cell Position
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A172 cells, Raji cells, Primary antibody: 122335(FAIM2 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A172 cells, Raji cells, Primary antibody: 122335(FAIM2 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 122335(FAIM2 Antibody) at dilution 1/30. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 122335(FAIM2 Antibody) at dilution 1/30. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 122335(FAIM2 Antibody) at dilution 1/30. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 122335(FAIM2 Antibody) at dilution 1/30. (Original magnification: ×200))
Product Categories/Family for anti-FAIM2/LFG antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
30,448 Da
NCBI Official Full Name
Homo sapiens Fas apoptotic inhibitory molecule 2, mRNA
NCBI Official Synonym Full Names
Fas apoptotic inhibitory molecule 2
NCBI Official Symbol
FAIM2
NCBI Official Synonym Symbols
LFG; LFG2; NGP35; NMP35; TMBIM2
NCBI Protein Information
protein lifeguard 2
UniProt Protein Name
Protein lifeguard 2
UniProt Gene Name
FAIM2
UniProt Synonym Gene Names
KIAA0950; LFG; LFG2; NMP35; TMBIM2
UniProt Entry Name
LFG2_HUMAN

Uniprot Description

FAIM2: Antiapoptotic protein which protects cells uniquely from Fas-induced apoptosis. Regulates Fas-mediated apoptosis in neurons by interfering with caspase-8 activation. May play a role in cerebellar development by affecting cerebellar size, internal granular layer (IGL) thickness, and Purkinje cell (PC) development. Belongs to the BI1 family. LFG subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Apoptosis

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: postsynaptic membrane; integral to membrane; cell junction; lipid raft

Biological Process: apoptosis; cerebellar granular layer development; regulation of neuron apoptosis; cerebellar Purkinje cell differentiation; cerebellar Purkinje cell layer development; cerebellum development; negative regulation of neuron apoptosis; negative regulation of apoptosis

Research Articles on FAIM2/LFG

Similar Products

Product Notes

The FAIM2/LFG faim2 (Catalog #AAA4754418) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FAIM2/LFG Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAIM2/LFG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the FAIM2/LFG faim2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAIM2/LFG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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