SDS-PAGE
(purified SIV gp120 protein (isolate SIVmac251v31523ru28))
SIV gp120 recombinant protein
SIV gp120 (Isolate SIVmac251v31523ru28) (aa 31-528)
Applications
Western Blot, ELISA
Purity
>= 95% purity(SDS-PAGE)
Synonyms
SIV gp120; SIV gp120 (Isolate SIVmac251v31523ru28) (aa 31-528); gp120 (SIV/mac251)(isolate SIVmac251v31523ru28); SIV Envelop Protein gp120 (Isolate SIVmac251v31523ru28); SIV gp120 recombinant protein
Host
293 cell culture
Purity/Purification
>= 95% purity(SDS-PAGE)
Concentration
1 mg/mL in PBS. (varies by lot)
Applicable Applications for SIV gp120 recombinant protein
Western Blot standard, antibody ELISA, HIV-1 entry inhibition, etc.
Application Notes
SDS-PAGE Data: purified SIV gp120 protein (isolate SIVmac251v31523ru28)
Viral Protein
6xHis tagged gp120 (SIV/mac251)(isolate SIVmac251v31523ru28) protein (amino acid 31-528) (Genebank accession#: GU952740)
Preparation and Storage
Store at -20°C. Stable for 3-months from the date of shipment when kept at 4 degree C. Non-hazardous. No MSDS required.
SDS-PAGE
(purified SIV gp120 protein (isolate SIVmac251v31523ru28))
SDS-PAGE
(purified SIV gp120 protein (isolate SIVmac251v31523ru28))
Related Product Information for SIV gp120 recombinant protein
6xHis tagged gp120 (SIV/mac251)(isolate SIVmac251v31523ru28) (aa 31-528)(Genebank No. GU952740)purified from 293 cell culture
Envelope protein gp120 expressed and purified from 293 cell culture.
Envelope glycoprotein GP120 (or gp120) is a glycoprotein exposed on the surface of the HIV envelope. The 120 in its name comes from its molecular weight of 120. Gp120 is essential for virus entry into cells as it plays a vital role in attachment to specific cell surface receptors. These receptors are DC-SIGN, Heparan Sulfate Proteoglycan and a specific interaction with the CD4 receptor, particularly on helper T-cells. Binding to CD4 induces the start of a cascade of conformational changes in gp120 and gp41 that lead to the fusion of the viral with the host cell membrane. Binding to CD4 is mainly electrostatic although there are van der Waals interactions and hydrogen bonds.
Envelope protein gp120 expressed and purified from 293 cell culture.
Envelope glycoprotein GP120 (or gp120) is a glycoprotein exposed on the surface of the HIV envelope. The 120 in its name comes from its molecular weight of 120. Gp120 is essential for virus entry into cells as it plays a vital role in attachment to specific cell surface receptors. These receptors are DC-SIGN, Heparan Sulfate Proteoglycan and a specific interaction with the CD4 receptor, particularly on helper T-cells. Binding to CD4 induces the start of a cascade of conformational changes in gp120 and gp41 that lead to the fusion of the viral with the host cell membrane. Binding to CD4 is mainly electrostatic although there are van der Waals interactions and hydrogen bonds.
Product Categories/Family for SIV gp120 recombinant protein
References
1. Stone, M., etc. A limited number of simian immunodeficiency virus (SIV) env variants are transmitted to rhesus macaques vaginally inoculated with SIVmac251. J. Virol. 84 (14), 7083-7095 (2010).
2. Manrique, M., etc. Resistance to infection, early and persistent suppression of SIVmac251 viremia and significant reduction of tissue viral burden after mucosal vaccination in females Rhesus macaques. J Virol. 2013 Oct 23
2. Manrique, M., etc. Resistance to infection, early and persistent suppression of SIVmac251 viremia and significant reduction of tissue viral burden after mucosal vaccination in females Rhesus macaques. J Virol. 2013 Oct 23
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Product Notes
The SIV gp120 (Catalog #AAA434203) is a Recombinant Protein produced from 293 cell culture and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SIV gp120 can be used in a range of immunoassay formats including, but not limited to, Western Blot standard, antibody ELISA, HIV-1 entry inhibition, etc. SDS-PAGE Data: purified SIV gp120 protein (isolate SIVmac251v31523ru28). Researchers should empirically determine the suitability of the SIV gp120 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIV gp120, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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