SDS-PAGE
(SDS-PAGE: purified HIV-1 gp120 (isolate MN) (Clade B) protein)
gp120 (MN) (HIV-1/Clade B) Recombinant Protein | gp120 recombinant protein
gp120 (MN) (HIV-1/Clade B) (aa 30-513)
Applications
Western Blot, ELISA
Purity
> 95% purity (SDS-PAGE)
Synonyms
gp120 (MN) (HIV-1/Clade B); gp120 (MN) (HIV-1/Clade B) (aa 30-513); glycosylated recombinant protein; gp120 (HIV-1/Clade B)(isolate MN); gp120 recombinant protein
Host
293 cell culture
Purity/Purification
> 95% purity (SDS-PAGE)
Concentration
1 mg/ml in PBS (varies by lot)
Applicable Applications for gp120 recombinant protein
Western Blot standard, antibody ELISA, HIV-1 entry inhibition
Viral Protein
C-terminal 6xHis tagged HIV-1 gp120 (isolate MN) (Clade B) protein (amino acid 30-513) (Genebank No. AF075722).
Preparation and Storage
Store at -20°C. Stable for 6-months from the date of shipment when kept at 4°C. Non-hazardous. No MSDS required.
SDS-PAGE
(SDS-PAGE: purified HIV-1 gp120 (isolate MN) (Clade B) protein)
SDS-PAGE
(SDS-PAGE: purified HIV-1 gp120 (isolate MN) (Clade B) protein)
Related Product Information for gp120 recombinant protein
Description: gp120 protein expressed and purified from 293 cell culture.
Introduction: Envelope glycoprotein GP120 (or gp120) is a glycoprotein exposed on the surface of the HIV envelope. The 120 in its name comes from its molecular weight of 120. Gp120 is essential for virus entry into cells as it plays a vital role in attachment to specific cell surface receptors. These receptors are DC-SIGN, Heparan Sulfate Proteoglycan and a specific interaction with the CD4 receptor, particularly on helper T-cells. Binding to CD4 induces the start of a cascade of conformational changes in gp120 and gp41 that lead to the fusion of the viral with the host cell membrane. Binding to CD4 is mainly electrostatic although there are van der Waals interactions and hydrogen bonds.
Introduction: Envelope glycoprotein GP120 (or gp120) is a glycoprotein exposed on the surface of the HIV envelope. The 120 in its name comes from its molecular weight of 120. Gp120 is essential for virus entry into cells as it plays a vital role in attachment to specific cell surface receptors. These receptors are DC-SIGN, Heparan Sulfate Proteoglycan and a specific interaction with the CD4 receptor, particularly on helper T-cells. Binding to CD4 induces the start of a cascade of conformational changes in gp120 and gp41 that lead to the fusion of the viral with the host cell membrane. Binding to CD4 is mainly electrostatic although there are van der Waals interactions and hydrogen bonds.
Product Categories/Family for gp120 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
HIV-1 isolate MN clone E6 from the USA, envelope glycoprotein (env) gene, complete cds
UniProt Protein Name
Envelope glycoprotein gp160
UniProt Gene Name
env
UniProt Entry Name
Q9YUL5_9HIV1
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Product Notes
The gp120 env (Catalog #AAA434051) is a Recombinant Protein produced from 293 cell culture and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's gp120 (MN) (HIV-1/Clade B) can be used in a range of immunoassay formats including, but not limited to, Western Blot standard, antibody ELISA, HIV-1 entry inhibition. Researchers should empirically determine the suitability of the gp120 env for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "gp120 (MN) (HIV-1/Clade B), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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