Sirtuin 6 (SIRT6) Recombinant Protein | SIRT6 recombinant protein

Recombinant Sirtuin 6 (SIRT6)

Cat. Num. AAA2009930

• Gene Names: Sirt6; Sir2l6; AI043036; 2810449N18Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Sirtuin 6 (SIRT6); Recombinant Sirtuin 6 (SIRT6); SIRT6 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-MSVNYAAGLS PYADKGKCGL PEIFDPPEEL ERKVWELARL MWQSSSVVFH TGAGISTASG IPDFRGPHGV WTMEERGLAP KFDTTFENAR PSKTHMALVQ LERMGFLSFL VSQNVDGLHV RSGFPRDKLA ELHGNMFVEE CPKCKTQYVR DTVVGTMGLK ATGRLCTVAK TRGLRACRGE LRDTILDWED SLPDRDLMLA DEASRTADLS VTLGTSLQIR PSGNLPLATK RRGGRLVIVN LQPTKHDRQA DLRIHGYVDE VMCRLMKHLG LEIPAWDGPC VLDKALPPLP RPVALKAEPP VHLNGAVHVS YKSKPNSPIL HRPPKRVKTE AAPS
• Sequence Length: 334

• Applicable Applications for SIRT6 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Ser334 (Accession # P59941) with N-terminal His-Tag
• Subcellular Location: Nucleus
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.9
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31712018
• NCBI GeneID: 50721
• NCBI Accession #: NP_853617.1
• NCBI GenBank Nucleotide #: NM_181586.3
• UniProt Accession #: P59941
• Molecular Weight: 38.4kDa
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-6 isoform 1
• NCBI Official Synonym Full Names: sirtuin 6
• NCBI Official Symbol: Sirt6
• NCBI Official Synonym Symbols: Sir2l6; AI043036; 2810449N18Rik
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-6; SIR2-like protein 6; regulatory protein SIR2 homolog 6; NAD-dependent deacetylase sirtuin-6
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-6
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: Sirt6
• UniProt Synonym Gene Names: Sir2l6
• UniProt Entry Name: SIR6_MOUSE

Uniprot Description

SIRT6: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.2.31; Deacetylase; EC 3.5.1.-; Transferase

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear telomeric heterochromatin; nucleus

Molecular Function: (3,5-dichlorophenylurea)acetate amidohydrolase activity; NAD(P)+-protein-arginine ADP-ribosyltransferase activity; hydrolase activity; metal ion binding; protein deacetylase activity; UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity; N-acetylgalactosamine-6-phosphate deacetylase activity; N2-acetyl-L-lysine deacetylase activity; iprodione amidohydrolase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); indoleacetamide hydrolase activity; protein N-terminal asparagine amidohydrolase activity; chromatin binding; histone deacetylase activity; O-succinylbenzoate synthase activity

Biological Process: protein amino acid ADP-ribosylation