Human NAD-dependent deacetylase sirtuin-6 ELISA Kit | SIRT6 elisa kit

Human NAD-dependent deacetylase sirtuin-6 ELISA Kit

Cat. Num. AAA2887118

• Gene Names: SIRT6; SIR2L6
• Reactivity: Human
• Synonyms: NAD-dependent deacetylase sirtuin-6; Human NAD-dependent deacetylase sirtuin-6 ELISA Kit; SIR2-like protein 6; SIRT6; SIR2L6; SIRT6 elisa kit

• Reactivity: Human
• Sequence Length: 328

• Assay Type: Competitive
• Detection Range: 78-5000 pg/mL
• Sensitivity: 20pg/mL
• Intra-Assay CV: <=5.2%
• Inter-Assay CV: <=10.3%
• Recovery: 113%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 300797597
• NCBI GeneID: 51548
• NCBI Accession #: NP_001180214.1
• NCBI GenBank Nucleotide #: NM_001193285.1
• UniProt Accession #: Q8N6T7; O75291; Q6IAF5; Q6PK99; Q8NCD2; Q9BSI5; Q9BWP3; Q9NRC7; Q9UQD1; B2RCD0
• Molecular Weight: 36,065 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-6 isoform 2
• NCBI Official Synonym Full Names: sirtuin 6
• NCBI Official Symbol: SIRT6
• NCBI Official Synonym Symbols: SIR2L6
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-6
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-6
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: SIRT6
• UniProt Synonym Gene Names: SIR2L6
• UniProt Entry Name: SIR6_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010]

Uniprot Description

SIRT6: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.2.31; Deacetylase; Transferase; EC 3.5.1.-

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; nuclear telomeric heterochromatin; nucleolus; nucleoplasm; nucleus

Molecular Function: chromatin binding; NAD(P)+-protein-arginine ADP-ribosyltransferase activity; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; transcription corepressor activity; zinc ion binding

Biological Process: base-excision repair; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; glucose homeostasis; histone deacetylation; negative regulation of cell proliferation; negative regulation of glucose import; negative regulation of glycolysis; negative regulation of transcription, DNA-dependent; positive regulation of fibroblast proliferation; protein amino acid ADP-ribosylation; protein destabilization; response to nutrient levels