Rabbit anti-Human SUMO-activating Enzyme Subunit 2 Polyclonal Antibody | anti-SAE2 antibody

SUMO-activating Enzyme Subunit 2, CT (E616) (Ubiquitin-like 1 Activating Enzyme E1B, Anthracycline-associated Resistance ARX, HRIHFB2115, SAE2, UBA2, UBLE1B) (Azide free) (HRP)

Cat. Num. AAA6501535

• Gene Names: UBA2; ARX; SAE2; HRIHFB2115
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SUMO-activating Enzyme Subunit 2; Polyclonal Antibody; CT (E616) (Ubiquitin-like 1 Activating Enzyme E1B; Anthracycline-associated Resistance ARX; HRIHFB2115; SAE2; UBA2; UBLE1B) (Azide free) (HRP); anti-SAE2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UBA2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SAE2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human UBA2 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-UBA2 C-term E616 Antibody is used in Western blot to detect UBA2 in Jurkat lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-SAE2 antibody

Antibodies; Ubiquitin

References

1. Desterro, J.M., et al., J. Biol. Chem. 274(15):10618-10624 (1999). 2. Gong, L., et al., FEBS Lett. 448(1):185-189 (1999). 3. Okuma, T., et al., Biochem. Biophys. Res. Commun. 254(3):693-698 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4885649
• NCBI GeneID: 10054
• NCBI Accession #: NP_005490
• NCBI GenBank Nucleotide #: NM_005499.2
• UniProt Accession #: Q9UBT2; O95605; Q59H87; Q6IBP6; Q9NTJ1; Q9UED2; B3KWB9
• Molecular Weight: 60,839 Da
• NCBI Official Full Name: SUMO-activating enzyme subunit 2
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 2
• NCBI Official Symbol: UBA2
• NCBI Official Synonym Symbols: ARX; SAE2; HRIHFB2115
• NCBI Protein Information: SUMO-activating enzyme subunit 2; SUMO1 activating enzyme subunit 2; SUMO-1 activating enzyme subunit 2; ubiquitin-like 1-activating enzyme E1B; anthracycline-associated resistance ARX; ubiquitin-like modifier-activating enzyme 2; UBA2, ubiquitin-activati
• UniProt Protein Name: SUMO-activating enzyme subunit 2
• Protein Family: SUMO-activating enzyme
• UniProt Gene Name: UBA2
• UniProt Synonym Gene Names: SAE2; UBLE1B
• UniProt Entry Name: SAE2_HUMAN

NCBI Description

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

Uniprot Description

SAE2: a protein of the ubiquitin-activating E1 family. Acts as a UBL1 E1 ligase. Mediates ATP-dependent activation of UBL1 and formation of a thiolester with a conserved cysteine residue on SAE2.

Protein type: Ligase; EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Motility/polarity/chemotaxis; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: SUMO activating enzyme activity; protein binding; protein heterodimerization activity; metal ion binding; enzyme activator activity; transcription factor binding; ATP binding

Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; SMT3-dependent protein catabolic process; post-translational protein modification