CD172g recombinant protein

CD172g Recombinant Protein

Cat. Num. AAA3004229

• Gene Names: SIRPG; CD172g; SIRPB2; SIRP-B2; bA77C3.1; SIRPgamma
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Synonyms: CD172g; CD172g Recombinant Protein; CD172g recombinant protein

• Host: E Coli
• Purity/Purification: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Form/Format: PBS, 4M Urea, PH7.4
• Concentration: >=0.5mg/ml (varies by lot)
• Sequence: EEELQMIQPEKLLLVTVGKTATLHCTVTSLLPVGPVLWFRGVGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRISSITPADVGTYYCVKFRKGSPENVEFKSGPGTEMALGAKPSAPVVLGPAARTTPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPTGQSVAYSIRSTARVVLDPWDVRSQVICEVAHVTLQGDPLRGTANLSEAIRVPPTLEVTQQPMRVGNQVNVTCQVRKFYPQSLQLTWSENGNVCQRETASTLTENKDGTYNWTSWFLVNISDQRDDVVLTCQVKHDGQLAVSKRLALEVTVHQKDQSSDATP

• Tag: His-tag in C-terminal
• Expression Vector: pet-22b(+)
• BP: 1008
• Restriction Site: NdeI-XhoI
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Testing Data

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Related Product Information for CD172g recombinant protein

Background: SIRPs (signal-regulatory proteins) are a family of transmembrane glycoproteins that were identified by their association with the Src homology 2 domaincontaining protein-tyrosine phosphatase SHP-2 in response to Insulin. The SIRP family negatively regulates the PI 3-K pathway, which may diminish EGFR-mediated motility and survival phenotypes that contribute to transformation of certain cell types. SIRP-alpha1 is a transmembrane protein which contains an extracellular portion with three immunoglobulin-like structures and a cytoplasmic region with four potential tyrosine phosphorylation sites. SIRP-alpha1 is a substrate for activated receptor tyrosine kinases. In its tyrosine phosphorylated form, SIRP-alpha1 binds to SH-PTP2 through SH2 interactions and acts as an SH-PTP2 substrate. SIRP-alpha1 has been shown to have negative regulatory effects on cellular responses induced by growth factors, oncogenes and insulin. SIRP-beta1 shares extensive sequence homology with SIRP-alpha1 in its extracellular portion but lacks the cytoplasmic portion. SIRP-gamma, originally designated SIRP-beta2 (SIRP-B2, CD172g) has unique characteristics from both the alpha and beta versions. SIRP-gamma is expressed on the majority of T cells and a proportion of B cells. CD47 associates with SIRP-gamma, and this interaction signals unidirectionally only.

NCBI and Uniprot Product Information

• NCBI GI #: 94538339
• NCBI GeneID: 55423
• NCBI Accession #: NP_001034597.1
• NCBI GenBank Nucleotide #: NM_001039508.1
• UniProt Accession #: Q9P1W8; Q5D051; Q5JV25; Q5MKL4; Q8WWA5; Q9NQK8; B1AKP6
• Molecular Weight: 42,498 Da
• NCBI Official Full Name: signal-regulatory protein gamma isoform 3
• NCBI Official Synonym Full Names: signal-regulatory protein gamma
• NCBI Official Symbol: SIRPG
• NCBI Official Synonym Symbols: CD172g; SIRPB2; SIRP-B2; bA77C3.1; SIRPgamma
• NCBI Protein Information: signal-regulatory protein gamma; SIRP-gamma; SIRP beta 2; SIRP-beta-2; CD172g antigen; signal-regulatory protein beta 2; signal-regulatory protein beta-2; CD172 antigen-like family member B
• UniProt Protein Name: Signal-regulatory protein gamma
• UniProt Gene Name: SIRPG
• UniProt Synonym Gene Names: SIRPB2; SIRP-gamma; SIRP-b2; SIRP-beta-2
• UniProt Entry Name: SIRPG_HUMAN

NCBI Description

The protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

SIRPG: Probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T- cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; cell adhesion; blood coagulation; leukocyte migration