Rabbit CD172g Polyclonal Antibody | anti-CD172g antibody

CD172g polyclonal antibody

Cat. Num. AAA3014912

• Gene Names: SIRPG; CD172g; SIRPB2; SIRP-B2; bA77C3.1; SIRPgamma
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: CD172g; Polyclonal Antibody; CD172g polyclonal antibody; Signal-regulatory protein gamma; SIRP-gamma; CD172 antigen-like family member B; Signal-regulatory protein beta-2; SIRP-b2; SIRP-beta-2; SIRPG; SIRPB2; anti-CD172g antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CD172g polyclonal antibody detects endogenous levels of CD172g protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD172g antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to Human CD172g
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of CD172g polyclonal antibody at 1:500 dilutionLaneA: AML-12 whole cell lysateLaneB: PC12 whole cell lysateLaneC: L02 whole cell lysateLaneD: HEK293T whole cell lysate)

Related Product Information for anti-CD172g antibody

SIRPs (signal-regulatory proteins) are a family of transmembrane glycoproteins that were identified by their association with the Src homology 2 domaincontaining protein-tyrosine phosphatase SHP-2 in response to Insulin. The SIRP family negatively regulates the PI 3-K pathway, which may diminish EGFR-mediated motility and survival phenotypes that contribute to transformation of certain cell types. SIRP-alpha1 is a transmembrane protein which contains an extracellular portion with three immunoglobulin-like structures and a cytoplasmic region with four potential tyrosine phosphorylation sites. SIRP-alpha1 is a substrate for activated receptor tyrosine kinases. In its tyrosine phosphorylated form, SIRP-alpha1 binds to SH-PTP2 through SH2 interactions and acts as an SH-PTP2 substrate. SIRP-alpha1 has been shown to have negative regulatory effects on cellular responses induced by growth factors, oncogenes and insulin. SIRP-beta1 shares extensive sequence homology with SIRP-alpha1 in its extracellular portion but lacks the cytoplasmic portion. SIRP-gamma, originally designated SIRP-beta2 (SIRP-B2, CD172g) has unique characteristics from both the alpha and beta versions. SIRP-gamma is expressed on the majority of T cells and a proportion of B cells. CD47 associates with SIRP-gamma, and this interaction signals unidirectionally only.

NCBI and Uniprot Product Information

• NCBI GI #: 94538339
• NCBI GeneID: 55423
• NCBI Accession #: NP_001034597.1
• NCBI GenBank Nucleotide #: NM_001039508.1
• UniProt Accession #: Q9P1W8; Q5D051; Q5JV25; Q5MKL4; Q8WWA5; Q9NQK8; B1AKP6
• Molecular Weight: 42,498 Da
• NCBI Official Full Name: signal-regulatory protein gamma isoform 3
• NCBI Official Synonym Full Names: signal-regulatory protein gamma
• NCBI Official Symbol: SIRPG
• NCBI Official Synonym Symbols: CD172g; SIRPB2; SIRP-B2; bA77C3.1; SIRPgamma
• NCBI Protein Information: signal-regulatory protein gamma; SIRP-gamma; SIRP beta 2; SIRP-beta-2; CD172g antigen; signal-regulatory protein beta 2; signal-regulatory protein beta-2; CD172 antigen-like family member B
• UniProt Protein Name: Signal-regulatory protein gamma
• UniProt Gene Name: SIRPG
• UniProt Synonym Gene Names: SIRPB2; SIRP-gamma; SIRP-b2; SIRP-beta-2
• UniProt Entry Name: SIRPG_HUMAN

NCBI Description

The protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

SIRPG: Probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T- cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; cell adhesion; blood coagulation; leukocyte migration