SIRP alpha / CD172a recombinant protein

Recombinant Mouse SIRP alpha / CD172a Protein (Fc tag)

Cat. Num. AAA2546723

• Gene Names: Sirpa; Bit; P84; SIRP; SHP-1; CD172a; Ptpns1; SHPS-1; AI835480
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: SIRP alpha / CD172a; Recombinant Mouse SIRP alpha / CD172a Protein (Fc tag); RP23-37B4.2; AI835480; Bit; CD172a; P84; Ptpns1; SHP-1; SHPS-1; SIRP; SIRP alpha / CD172a recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 509

• Application Notes: The secreted recombinant mouse SIRPA/Fc is a disulfide-linked homodimer. The reduced monomer comprises 583 amino acids and has a calculated molecular mass of 64.9 kDa. As a result of glycosylation, the apparent molecular mass of rmSIRPA/Fc monomer is approximately 1001-20 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Thr 32
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for SIRP alpha / CD172a recombinant protein

Background: Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

Description: A DNA sequence encoding the extracellular domain of mouse SIRPA (BAA20376.1) (Met 1-Asn 373) was fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 2190257
• NCBI GeneID: 19261
• Molecular Weight: 32,145 Da
• NCBI Official Full Name: BIT
• NCBI Official Synonym Full Names: signal-regulatory protein alpha
• NCBI Official Symbol: Sirpa
• NCBI Official Synonym Symbols: Bit; P84; SIRP; SHP-1; CD172a; Ptpns1; SHPS-1; AI835480
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type substrate 1
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type substrate 1
• UniProt Gene Name: Sirpa
• UniProt Synonym Gene Names: Bit; Myd1; Ptpns1; Shps1; Sirp; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1; mSIRP-alpha1

Uniprot Description

SHPS1: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B. Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 2 F1|2 63.19 cM

Cellular Component: integral to plasma membrane

Molecular Function: protein binding; protein phosphorylated amino acid binding

Biological Process: actin filament organization; cell migration; cell motility; cell-matrix adhesion; cytoskeleton organization and biogenesis; hemopoietic progenitor cell differentiation; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of phagocytosis