Goat PDE4D Polyclonal Antibody | anti-PDE4D antibody

Goat Polyclonal to Human PDE4D

Cat. Num. AAA243463

• Gene Names: PDE4D; DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey, Bat, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: PDE4D; Polyclonal Antibody; Goat Polyclonal to Human PDE4D; Anti-PDE4D Antibody (C-Terminus) IHC-plus; ACRDYS2; DPDE3; HSPDE4D; PDE43; Pde-ivd; PDE4DN2; Phosphodiesterase 4d; STRK1; Human PDE4D; anti-PDE4D antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey, Bat, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human PDE4D. This antiobody is expected to recognize all reported isoforms.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 673

• Applicable Applications for anti-PDE4D antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (5 ug/ml), WB (0.1 - 1 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide QPEACVIDDRSPDT from the C-terminus of human PDE4D (NP_001184151.1; NP_001184150.1; NP_006194.2; NP_001098101.1; NP_001184152.1; NP_001159371.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: PDE4D antibody was raised against synthetic peptide QPEACVIDDRSPDT from the C-terminus of human PDE4D (NP_001184151.1; NP_001184150.1; NP_006194.2; NP_001098101.1; NP_001184152.1; NP_001159371.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Monkey, Bat, Rabbit (93%); Bovine (86%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PDE4D antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-PDE4D antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody staining (1 ug/ml) of COS cell lysates (25 ug protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-PDE4D antibody

Phosphodiesterase 4D, a cAMP-specific phosphodiesterase, has been shown to affect growth and fertility, functioning of pro-inflammatory immune cells, and cholinergic stimulation of the airway and consequently the development of hyperreactive disease states such as asthma. PDE4D activity is inhibited by rolipram. At least six splice variants have been reported.

Product Categories/Family for anti-PDE4D antibody

Family: Phosphodiesterase; Subfamily: PDE - cAMP-specific

NCBI and Uniprot Product Information

• NCBI GI #: 32306513
• NCBI GeneID: 5144
• NCBI Accession #: NP_006194.2
• NCBI GenBank Nucleotide #: NM_006203.4
• UniProt Accession #: Q08499; O43433; Q13549; Q13550; Q13551; Q7Z2L8; Q8IV84; Q8IVA9; Q8IVD2; Q8IVD3; Q96HL4; Q9HCX7
• Molecular Weight: 24,429 Da
• NCBI Official Full Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform PDE4D3
• NCBI Official Synonym Full Names: phosphodiesterase 4D, cAMP-specific
• NCBI Official Symbol: PDE4D
• NCBI Official Synonym Symbols: DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
• NCBI Protein Information: cAMP-specific 3',5'-cyclic phosphodiesterase 4D; cAMP-specific phosphodiesterase PDE4D6; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• UniProt Protein Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4D
• Protein Family: cAMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE4D
• UniProt Synonym Gene Names: DPDE3
• UniProt Entry Name: PDE4D_HUMAN

NCBI Description

This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]

Uniprot Description

PDE4D: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. Inhibited by rolipram. Activated by phosphatidic acid. Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; EC 3.1.4.53; Phosphodiesterase

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: Golgi apparatus; centrosome; membrane; myofibril; apical plasma membrane; voltage-gated calcium channel complex; cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; protein binding; enzyme binding; ubiquitin protein ligase binding; metal ion binding; beta-2 adrenergic receptor binding; drug binding; cAMP binding; 3',5'-cyclic-nucleotide phosphodiesterase activity; SH3 domain binding; ATPase binding

Biological Process: neutrophil chemotaxis; negative regulation of heart contraction; regulation of heart rate; multicellular organism growth; positive regulation of smooth muscle cell proliferation; cAMP catabolic process; negative regulation of peptidyl-serine phosphorylation; positive regulation of smooth muscle cell migration; T cell receptor signaling pathway; memory; cAMP-mediated signaling; positive regulation of interferon-gamma production; smooth muscle contraction; cellular protein complex assembly; positive regulation of interleukin-2 production; regulation of receptor activity; positive regulation of interleukin-5 production; regulation of G-protein coupled receptor protein signaling pathway; aging; lung development