Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human SIGLEC5(CD170) expression on human whole blood granulocytes. Cells were stained with FITC-conjugated anti-Human SIGLEC5(CD170). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.)
Rabbit anti-Mouse SIRP alpha Monoclonal Antibody | anti-SIRPA antibody
Recombinant Anti-SIRP alpha Antibody (FITC), Rabbit Monoclonal
Gene Names
Sirpa; Bit; P84; SIRP; SHP-1; CD172a; Ptpns1; SHPS-1; AI835480
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
SIRP alpha; Monoclonal Antibody; Recombinant Anti-SIRP alpha Antibody (FITC); Rabbit Monoclonal; SIRP alpha/CD172a Antibody (FITC); Rabbit MAb; signal-regulatory protein alpha; Anti-AI835480 Antibody; Anti-Bit Antibody; Anti-CD172a Antibody; Anti-P84 Antibody; Anti-Ptpns1 Antibody; Anti-SHP-1 Antibody; Anti-SHPS-1 Antibody; Anti-SIRP Antibody; anti-SIRPA antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
001
Specificity
Mouse SIRP alpha/CD172a
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
5ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-SIRPA antibody
Flow Cytometry (FC/FACS/FCM)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse SIRP alpha/CD172a Protein
Conjugation
FITC
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse SIRP alpha/CD172a (rM SIRP alpha/CD172a) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human SIGLEC5(CD170) expression on human whole blood granulocytes. Cells were stained with FITC-conjugated anti-Human SIGLEC5(CD170). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human SIGLEC5(CD170) expression on human whole blood granulocytes. Cells were stained with FITC-conjugated anti-Human SIGLEC5(CD170). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.)
Related Product Information for anti-SIRPA antibody
Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.
Product Categories/Family for anti-SIRPA antibody
References
Timms JF. et al., 1999, Curr Biol. 9: 927-30. Stofega MR. et al., 2000, J Biol Chem. 275: 28222-9. Liu T. et al., 2005, J Proteome Res. 4: 2070-80. Wolf-Yadlin A. et al., 2007, Proc Natl Acad Sci. 104: 5860-5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
32,145 Da
NCBI Official Full Name
BIT
NCBI Official Synonym Full Names
signal-regulatory protein alpha
NCBI Official Symbol
Sirpa
NCBI Official Synonym Symbols
Bit; P84; SIRP; SHP-1; CD172a; Ptpns1; SHPS-1; AI835480
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type substrate 1
Protein Family
Research Articles on SIRPA
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Product Notes
The SIRPA (Catalog #AAA8112187) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-SIRP alpha Antibody (FITC), Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SIRP alpha can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the SIRPA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRP alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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