Single Ig IL1 Related Receptor (SIGIRR) Recombinant Protein | SIGIRR recombinant protein

Recombinant Single Ig IL1 Related Receptor (SIGIRR)

Cat. Num. AAA2010695

• Gene Names: Sigirr; RGD1306732
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Single Ig IL1 Related Receptor (SIGIRR); Recombinant Single Ig IL1 Related Receptor (SIGIRR); SIGIRR recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-MAGVCDRVPN FLSPSEDQAL GPALGSAVAL NCTAWVFSRP QCPQPSVQWL KDGLALGNGS HFSLHQDFWV SDNFSEIVSS VLVFNLTKAE DYGTFTCSAW NVSSHSFTLW RAGPAGHV
• Sequence Length: 409

• Applicable Applications for SIGIRR recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Val118 (Accession # Q4V892) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 67846050
• NCBI GeneID: 309106
• NCBI Accession #: NP_001020058.1
• NCBI GenBank Nucleotide #: NM_001024887.1
• UniProt Accession #: Q4V892
• Molecular Weight: 14.3kDa
• NCBI Official Full Name: single Ig IL-1-related receptor
• NCBI Official Synonym Full Names: single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
• NCBI Official Symbol: Sigirr
• NCBI Official Synonym Symbols: RGD1306732
• NCBI Protein Information: single Ig IL-1-related receptor; TIR8; toll/interleukin-1 receptor 8; single Ig IL-1R-related molecule; single immunoglobulin domain-containing IL1R-related protein
• UniProt Protein Name: Single Ig IL-1-related receptor
• Protein Family: Single Ig IL-1-related receptor
• UniProt Gene Name: Sigirr
• UniProt Synonym Gene Names: TIR8
• UniProt Entry Name: SIGIR_RAT

Uniprot Description

Function: Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP

By similarity.

Subunit structure: Interacts with IL1R1, IRAK1, TLR4, TLR5, TLR9 and TRAF6. Upon IL-1 stimulation found in a complex at least composed of IL1R1, SIGIRR, MYD88, IRAK1 and TRAF6. Upon stimulation with LPC found in a complex at least composed of TLR4, SIG1IR, MYD88, IRAK1 and TRAF6. Interacts with PALM3

By similarity.

Subcellular location: Membrane; Single-pass type III membrane protein

Potential.

Sequence similarities: Belongs to the interleukin-1 receptor family.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 TIR domain.