Western Blot/Blotting
(Western blot using ROBO1 antibody on human brain lysate . Lysate used at 15 ug/lane. Antibody used at 10 ug/ml. Secondary antibody, mouse anti-rabbit HRP , used at 1:50k.)
Rabbit anti-Human Roundabout homolog 1, ROBO1 Polyclonal Antibody | anti-ROBO1 antibody
Roundabout homolog 1, ROBO1
Western Blot/Blotting
(Western blot using ROBO1 antibody on human brain lysate . Lysate used at 15 ug/lane. Antibody used at 10 ug/ml. Secondary antibody, mouse anti-rabbit HRP , used at 1:50k.)
Western Blot/Blotting
(Western blot using ROBO1 antibody on human brain lysate . Lysate used at 15 ug/lane. Antibody used at 10 ug/ml. Secondary antibody, mouse anti-rabbit HRP , used at 1:50k.)
NCBI and Uniprot Product Information
NCBI Description
Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Uniprot Description
Function: Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. Ref.6
Subunit structure: Interacts with SLIT1 and SLIT2. Ref.6 Ref.10
Subcellular location: Membrane; Single-pass type I membrane protein
Potential.
Tissue specificity: Widely expressed, with exception of kidney. Ref.7
Post-translational modification: Ubiquitinated. May be deubiquitinated by USP33
By similarity.
Miscellaneous: Maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line. The promoter region of ROBO1 shows complete hypermethylation of CpG sites in the BT-20 breast tumor cell lines, some primary invasive breast carcinomasa and some primary clear cell renal cell carcinomas (CC-RCC).
Sequence similarities: Belongs to the immunoglobulin superfamily. ROBO family.Contains 3 fibronectin type-III domains.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Research Articles on ROBO1
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Product Notes
The ROBO1 robo1 (Catalog #AAA395980) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Roundabout homolog 1, ROBO1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Roundabout homolog 1, ROBO1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the ROBO1 robo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Roundabout homolog 1, ROBO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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