Rat Small heterodimer partner ELISA Kit | SHP elisa kit

Rat Small heterodimer partner ELISA Kit

Cat. Num. AAA720768

• Gene Names: NR0B2; SHP; SHP1
• Reactivity: Rat
• Synonyms: Small heterodimer partner; Rat Small heterodimer partner ELISA Kit; SHP elisa kit

• Reactivity: Rat

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SHP elisa kit

Intended Uses: This SCCAg2 ELISA kit is intended Laboratory for research use only. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of SCCAg2 in the sample, this SCCAg2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus SCCAg2 concentration. The concentration of SCCAg2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This SCCAg2 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for SCCAg2. Standards or samples are then added to the microtiter plate wells and SCCAg2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SCCAg2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SCCAg2 are added to each well to "sandwich" the SCCAg2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SCCAg2 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 15787843
• NCBI GeneID: 8431
• Molecular Weight: 28,058 Da
• NCBI Official Full Name: small heterodimer partner
• NCBI Official Synonym Full Names: nuclear receptor subfamily 0, group B, member 2
• NCBI Official Symbol: NR0B2
• NCBI Official Synonym Symbols: SHP; SHP1
• NCBI Protein Information: nuclear receptor subfamily 0 group B member 2; nuclear receptor SHP; small heterodimer partner; orphan nuclear receptor SHP
• UniProt Protein Name: Nuclear receptor subfamily 0 group B member 2
• Protein Family: Head decoration protein
• UniProt Gene Name: NR0B2
• UniProt Synonym Gene Names: SHP
• UniProt Entry Name: NR0B2_HUMAN

NCBI Description

The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. [provided by RefSeq, Jul 2008]

Uniprot Description

NR0B2: Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box- containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1. Defects in NR0B2 may be associated with obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the nuclear hormone receptor family. NR0 subfamily.

Protein type: Nuclear receptor; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p36.1

Cellular Component: nucleoplasm; protein complex; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; protein domain specific binding; peroxisome proliferator activated receptor binding; protein binding; protein homodimerization activity; DNA binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor binding; transcription corepressor activity; transcription factor activity

Biological Process: cholesterol metabolic process; transcription initiation from RNA polymerase II promoter; organ regeneration; negative regulation of transcription factor activity; response to glucose stimulus; positive regulation of insulin secretion; steroid hormone mediated signaling; gene expression; negative regulation of transcription from RNA polymerase II promoter

Disease: Obesity