Human Small Heterodimer Partner (SHP) ELISA Kit | SHP elisa kit

Human Small Heterodimer Partner (SHP) ELISA Kit

Cat. Num. AAA2020499

• Gene Names: NR0B2; SHP; SHP1
• Reactivity: Human
• Synonyms: Small Heterodimer Partner (SHP); Human Small Heterodimer Partner (SHP) ELISA Kit; NR0B2; SHP1; Nuclear Receptor Subfamily 0; Group B; Member 2; Orphan nuclear receptor SHP; SHP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Small Heterodimer Partner (SHP).; No significant cross-reactivity or interference between Small Heterodimer Partner (SHP) and analogues was observed.
• Sequence Length: 257

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Small Heterodimer Partner (SHP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Small Heterodimer Partner (SHP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SHP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Heterodimer Partner (SHP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Small Heterodimer Partner (SHP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Heterodimer Partner (SHP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Small Heterodimer Partner (SHP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SHP elisa kit

Signal transduction; Endocrinology; Developmental science; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 15787843
• NCBI GeneID: 8431
• UniProt Accession #: Q15466; Q5QP36; F1D8P5
• Molecular Weight: 28,058 Da
• NCBI Official Full Name: small heterodimer partner
• NCBI Official Synonym Full Names: nuclear receptor subfamily 0, group B, member 2
• NCBI Official Symbol: NR0B2
• NCBI Official Synonym Symbols: SHP; SHP1
• NCBI Protein Information: nuclear receptor subfamily 0 group B member 2
• UniProt Protein Name: Nuclear receptor subfamily 0 group B member 2
• Protein Family: Head decoration protein
• UniProt Gene Name: NR0B2
• UniProt Synonym Gene Names: SHP
• UniProt Entry Name: NR0B2_HUMAN

NCBI Description

The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. [provided by RefSeq, Jul 2008]

Uniprot Description

NR0B2: Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box- containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1. Defects in NR0B2 may be associated with obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the nuclear hormone receptor family. NR0 subfamily.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Nuclear receptor

Chromosomal Location of Human Ortholog: 1p36.1

Cellular Component: nucleoplasm; protein complex; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; protein domain specific binding; protein binding; peroxisome proliferator activated receptor binding; protein homodimerization activity; DNA binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor binding; transcription factor activity; transcription corepressor activity

Biological Process: cholesterol metabolic process; transcription initiation from RNA polymerase II promoter; organ regeneration; negative regulation of transcription factor activity; response to glucose stimulus; positive regulation of insulin secretion; gene expression; steroid hormone mediated signaling; negative regulation of transcription from RNA polymerase II promoter

Disease: Obesity