Western Blot (WB)
(Western blot of Alkaline Phosphatase conjugated Sheep Anti-Rabbit IgG. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. None. Alkaline Phosphatase sheep secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..)
Sheep anti Rabbit IgG (H + L) (Alk Phos) Secondary Antibody | IgG secondary antibody
Sheep anti Rabbit IgG (H + L) (Alk Phos)
Reactivity
Rabbit IgG (H + L)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Sheep anti Rabbit IgG (H + L) (Alk Phos) was purified by affinity chromatography.
Synonyms
Sheep anti Rabbit IgG (H + L) (Alk Phos); Sheep anti Rabbit Immunoglobulin G (H + L) secondary (Alk Phos); IgG secondary antibody
Host
Sheep
Reactivity
Rabbit IgG (H + L)
Specificity
Rabbit IgG (H + L)
Purity/Purification
Sheep anti Rabbit IgG (H + L) (Alk Phos) was purified by affinity chromatography.
Form/Format
Sterile filtered liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001 M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, with 10mglml BSA (Immunoglobulin and Protease free), and 0.01 % Sodium Azide as preservative.
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for IgG secondary antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:15,000
IHC: 1:200-1:1,000
WB: 1:500-1:2,000
IHC: 1:200-1:1,000
WB: 1:500-1:2,000
Immunogen
Rabbit IgG whole molecule
Tag/Conjugate
Alkaline Phosphatase
Cross-Reactivity
Assay by IEP resulted in a single precipitin arc against anti-Alkiline Phosphatase (calf intestine), anti-sheep serum, Rabbit IgG and Rabbit serum.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Biological Significance
Immunoglobulin G (lgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer
Preparation and Storage
4°C DO NOT FREEZE. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in substantial loss of enzymatic activity.
Western Blot (WB)
(Western blot of Alkaline Phosphatase conjugated Sheep Anti-Rabbit IgG. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. None. Alkaline Phosphatase sheep secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..)
Western Blot (WB)
(Western blot of Alkaline Phosphatase conjugated Sheep Anti-Rabbit IgG. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. None. Alkaline Phosphatase sheep secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..)
Related Product Information for IgG secondary antibody
Sheep anti Rabbit IgG (H + L) secondary antibody (Alk Phos)
Product Categories/Family for IgG secondary antibody
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Product Notes
The IgG (Catalog #AAA538626) is a Secondary Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Sheep anti Rabbit IgG (H + L) (Alk Phos) reacts with Rabbit IgG (H + L) and may cross-react with other species as described in the data sheet. AAA Biotech's Sheep anti Rabbit IgG (H + L) (Alk Phos) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:15,000 IHC: 1:200-1:1,000 WB: 1:500-1:2,000. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sheep anti Rabbit IgG (H + L) (Alk Phos), Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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