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SDS-Page

ANAPC13 recombinant protein

ANAPC13, 1-74aa, Human, T7 tag, E Coli

Gene Names
ANAPC13; SWM1; APC13
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
ANAPC13; 1-74aa; Human; T7 tag; E Coli; Anaphase-promoting complex subunit 13; APC13; SWM1; ANAPC13 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MASMTGGQQM GRGSHMDSEV QRDGRILDLI DDAWREDKLP YEDVAIPLNE LPEPEQDNGG TTESVKEQEM KWTDLALQYL HENVPPIGN
Sequence Length
74
Applicable Applications for ANAPC13 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
T7 tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for ANAPC13 recombinant protein
ANAPC13, also known as anaphase-promoting complex subunit 13, is component of the anaphase promoting complex, a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. This protein is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the anaphase promoting complex Recombinant human ANAPC13 protein fused to T7-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for ANAPC13 recombinant protein
References
Schwickart M., et al. (2004) Mol. Cell. Biol. 24:3562-3576; Jin L., et al. (2008) Cell. 133:653-665

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10.0 kDa (89aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
NCBI Official Full Name
anaphase-promoting complex subunit 13
NCBI Official Synonym Full Names
anaphase promoting complex subunit 13
NCBI Official Symbol
ANAPC13
NCBI Official Synonym Symbols
SWM1; APC13
NCBI Protein Information
anaphase-promoting complex subunit 13
UniProt Protein Name
Anaphase-promoting complex subunit 13
UniProt Gene Name
ANAPC13
UniProt Synonym Gene Names
APC13

NCBI Description

This gene encodes a component of the anaphase promoting complex, a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. The encoded protein is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the anaphase promoting complex. Pseudogenes and splice variants have been found for this gene; however, the biological validity of some of the splice variants has not been determined. [provided by RefSeq, Nov 2008]

Uniprot Description

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Research Articles on ANAPC13

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Product Notes

The ANAPC13 anapc13 (Catalog #AAA204480) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is T7 tag. AAA Biotech's ANAPC13 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the ANAPC13 anapc13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MASMTGGQQM GRGSHMDSEV QRDGRILDLI DDAWREDKLP YEDVAIPLNE LPEPEQDNGG TTESVKEQEM KWTDLALQYL HENVPPIGN. It is sometimes possible for the material contained within the vial of "ANAPC13, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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