Sheep anti-Mouse Nepmucin Antibody
Nepmucin (CD300LG, a not expressed in Peyer's Patch mucin, CLM-9) (Biotin)
Reactivity
Mouse
Applications
Western Blot
Purity
Purified by immunoaffinity chromatography
Synonyms
Nepmucin; Nepmucin (CD300LG; a not expressed in Peyer's Patch mucin; CLM-9) (Biotin); Anti -Nepmucin (CD300LG; anti-Nepmucin antibody
Host
Sheep
Reactivity
Mouse
Isotype
IgG
Specificity
Recognizes mouse Nepmucin. Shows less than 1% crossreactivity with recombinant human Nepmucin
Purity/Purification
Purified by immunoaffinity chromatography
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 50ug BSA.Reconstitute with 250ul sterile PBS
Concentration
~0.2mg/ml (after reconstitution) (varies by lot)
Applicable Applications for anti-Nepmucin antibody
Western Blot (WB)
Application Notes
Western Blot:0.1ug/ml
Optimal dilutions to be determined by the researcher. Other applications not tested.
Optimal dilutions to be determined by the researcher. Other applications not tested.
Grade
Affinity Purified
Immunogen
Recombinant protein corresponding to Leu19-Ile284 from mouseNepmucin expressed in NS0 cells
Preparation and Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Nepmucin antibody
Nepmucin (a not expressed in Peyer's Patch mucin; also CLM-9) is an 80-95kD member of the CD300L family of proteins. It is expressed on endothelium and serves multiple functions. It is known to form homotypic interactions between endothelial cells, bind lymphocytes via its Ig-like and mucin domains and serve as a transcellular transport vehicle for IgA and IgM. Mature mouse Nepmucin is a 397 amino acid (aa) type I transmembrane glycoprotein. Its extracellular region (aa 19-288) contains one V-type Ig-like domain (aa 19-124) plus an O-glycosylated mucin region (aa 136-276). There are three isoform variants. One is 60-75kD in size and shows an Ala substitution for aa 126-170, a second is 46-50kD in size and shows an Arg substitution for aa 170-254, while a third is 36kD in size and shows a SerTyr substitution for aa 134-256. Over aa 1-284, mouse Nepmucin shares 89% and 53% aa identity with rat and human Nepmucin, respectively.
Product Categories/Family for anti-Nepmucin antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
nepmucin isoform D
Uniprot Description
CD300LG: Receptor which may mediate L-selectin-dependent lymphocyte rollings. Binds SELL in a calcium dependent manner. Binds lymphocyte. Belongs to the CD300 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: membrane; basolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; endosome
Molecular Function: IgM binding
Biological Process: immune system process; immunoglobulin transcytosis in epithelial cells
Similar Products
Product Notes
The Nepmucin (Catalog #AAA619615) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Nepmucin (CD300LG, a not expressed in Peyer's Patch mucin, CLM-9) (Biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nepmucin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot:0.1ug/ml Optimal dilutions to be determined by the researcher. Other applications not tested. Researchers should empirically determine the suitability of the Nepmucin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nepmucin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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