SDS-Page
(Detection of MCAM: Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-mMCAM.)
Sheep anti-Mouse MCAM Polyclonal Antibody | anti-MCAM antibody
MCAM (Cell Surface Glycoprotein MUC18, Melanoma-associated Antigen MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, S-endo 1 Endothelial-associated Antigen, Cell Surface Glycoprotein P1H12, CD146, MCAM, MUC18)
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
MCAM; Polyclonal Antibody; MCAM (Cell Surface Glycoprotein MUC18; Melanoma-associated Antigen MUC18; Melanoma Cell Adhesion Molecule; Melanoma-associated Antigen A32; S-endo 1 Endothelial-associated Antigen; Cell Surface Glycoprotein P1H12; CD146; MUC18); Anti -MCAM (Cell Surface Glycoprotein MUC18; anti-MCAM antibody
Host
Sheep
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse MCAM. Species sequence homology: rat (90%), human (74%).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-MCAM antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Immunogen
NS0-derived, recombinant mouse MCAM extracellular domain (rmMCAM; aa24-563; Accession # Q8R2Y2).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of MCAM: Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-mMCAM.)
SDS-Page
(Detection of MCAM: Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-mMCAM.)
Related Product Information for anti-MCAM antibody
MCAM (Melanoma cell adhesion molecule; also CD146 and MUC18) is a 110-120kD member of a small group of Ig-superfamily molecules that includes CD239 and CD166. MCAM has also been reported at a molecular weight of approximately 150kD. In rodent, MCAM is reportedly expressed on neurons, endothelial cells, NK cells, neutrophils, mesenchymal stem cells and melanoma cells. MCAM appears to contribute to intercellular endothelial cell junctions, and possibly contributes to the migration of select cell types. Mature mouse MCAM is a 625aa type I transmembrane glycoprotein. Its extracellular region is 540aa in length (aa24-563). It contains two V-type Ig-like domains (aa24-244) followed by three C2-type Ig-like domains (aa246-512). One cytoplasmic region splice form shows a sevenaa substitution for aa600-648. Unlike human, rodent MCAM does not undergo a splicing event that will generate a soluble isoform. Over aa24-563, mouse MCAM shares 90% and 74% aa identity with rat and human MCAM, respectively.
Product Categories/Family for anti-MCAM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,075 Da
NCBI Official Full Name
melanoma cell adhesion molecule
NCBI Official Symbol
MCAM
NCBI Protein Information
melanoma cell adhesion molecule; HEMCAM; gicerin
UniProt Protein Name
L-gicerin
Protein Family
UniProt Entry Name
Q90880_CHICK
Research Articles on MCAM
Similar Products
Product Notes
The MCAM (Catalog #AAA628077) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The MCAM (Cell Surface Glycoprotein MUC18, Melanoma-associated Antigen MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, S-endo 1 Endothelial-associated Antigen, Cell Surface Glycoprotein P1H12, CD146, MCAM, MUC18) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MCAM can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.5-1ug/ml Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the MCAM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCAM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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