Typical Testing Data/Standard Curve (for reference only)
Sheep Vascular Endothelial cell growth factor (VEGF) ELISA Kit | VEGF elisa kit
Sheep Vascular Endothelial cell growth factor (VEGF) ELISA Kit
Gene Names
VEGFA; VEGF
Reactivity
Sheep
Synonyms
Vascular Endothelial cell growth factor (VEGF); Sheep Vascular Endothelial cell growth factor (VEGF) ELISA Kit; VEGF elisa kit
Reactivity
Sheep
Sequence Length
173
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
5-2000ng/L
Sensitivity
2.46ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for VEGF elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Sheep Vascular Endothelial cell growth factor (also known as VEGF) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Sheep VEGF antibody. VEGF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Sheep VEGF Antibody is added and binds to VEGF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VEGF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Sheep VEGF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Sheep VEGF antibody. VEGF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Sheep VEGF Antibody is added and binds to VEGF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VEGF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Sheep VEGF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,368 Da
NCBI Official Full Name
VEGF, partial
NCBI Official Symbol
VEGFA
NCBI Official Synonym Symbols
VEGF
NCBI Protein Information
vascular endothelial growth factor A
UniProt Protein Name
Vascular endothelial growth factor A
UniProt Gene Name
VEGFA
UniProt Synonym Gene Names
VEGF; VEGF-A; VPF
Uniprot Description
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development ().
Research Articles on VEGF
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Product Notes
The Sheep VEGF vegfa (Catalog #AAA1602118) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1602118 ELISA Kit recognizes Sheep VEGF. It is sometimes possible for the material contained within the vial of "Vascular Endothelial cell growth factor (VEGF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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