Sheep HNRPU ELISA Kit | HNRPU elisa kit

Sheep HNRPU (Heterogeneous Nuclear Ribonucleoprotein U) ELISA Kit

Cat. Num. AAA2505988

• Gene Names: HNRNPU; HNRPU; SAF-A; U21.1; hnRNP U
• Reactivity: Sheep
• Synonyms: HNRPU; Sheep HNRPU (Heterogeneous Nuclear Ribonucleoprotein U) ELISA Kit; HNRPU elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes natural and recombinant Sheep HNRPU. No significant cross-reactivity or interference between Sheep HNRPU and analogues was observed.
• Sequence Length: 825

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HNRPU elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep HNRPU concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HNRPU. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HNRPU and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HNRPU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HNRPU. You can calculate the concentration of HNRPU in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 74136883
• NCBI GeneID: 3192
• NCBI Accession #: NP_114032.2
• NCBI GenBank Nucleotide #: NM_031844.2
• UniProt Accession #: Q00839; O75507; Q8N174; Q96HY9; Q9BQ09
• Molecular Weight: 88,980 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein U isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
• NCBI Official Symbol: HNRNPU
• NCBI Official Synonym Symbols: HNRPU; SAF-A; U21.1; hnRNP U
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein U; p120 nuclear protein; pp120; scaffold attachment factor A
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein U
• UniProt Gene Name: HNRNPU
• UniProt Synonym Gene Names: HNRPU; SAFA; U21.1; hnRNP U; SAF-A
• UniProt Entry Name: HNRPU_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP U: Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single- stranded DNA and RNA. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Spliceosome; RNA splicing; Nuclear receptor co-regulator; RNA-binding

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: nucleoplasm; cell surface; membrane; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; DNA binding; RNA binding; ATP binding

Biological Process: osteoblast differentiation; nuclear mRNA splicing, via spliceosome; RNA processing; RNA splicing; gene expression; circadian regulation of gene expression