Human Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) ELISA Kit | HNRPU elisa kit

Human Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) ELISA Kit

Cat. Num. AAA450291

• Gene Names: HNRNPU; SAFA; HNRPU; SAF-A; U21.1; hnRNP U; HNRNPU-AS1
• Reactivity: Human
• Synonyms: Heterogeneous Nuclear Ribonucleoprotein U (HNRPU); Human Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) ELISA Kit; hnRNP-U; HNRNPU; SAF-A; U21.1; Scaffold Attachment Factor A; HNRPU elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HNRPU. No significant cross-reactivity or interference between HNRPU and analogues was observed.
• Sequence Length: 806

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HNRPU were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HNRPU were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HNRPU elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HNRPU in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HNRPU. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HNRPU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HNRPU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HNRPU in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14141161
• NCBI GeneID: 3192
• NCBI Accession #: NP_004492.2
• NCBI GenBank Nucleotide #: NM_004501.3
• UniProt Accession #: Q00839; O75507; Q8N174; Q96HY9; Q9BQ09
• Molecular Weight: 88,980 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein U isoform b
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein U
• NCBI Official Symbol: HNRNPU
• NCBI Official Synonym Symbols: SAFA; HNRPU; SAF-A; U21.1; hnRNP U; HNRNPU-AS1
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein U
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein U
• UniProt Gene Name: HNRNPU
• UniProt Synonym Gene Names: HNRPU; SAFA; U21.1; hnRNP U; SAF-A

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA. Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription ().