Typical Testing Data/Standard Curve (for reference only)
Rat DRD4 ELISA Kit | DRD4 elisa kit
Rat DRD4 (Dopamine Receptor D4) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat DRD4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat DRD4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DRD4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat DRD4. You can calculate the concentration of Rat DRD4 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats. [provided by RefSeq, Jul 2008]
Uniprot Description
DRD4: Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Its activity is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family.
Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: membrane; cell soma; integral to plasma membrane; plasma membrane; dendritic spine; terminal button; cell cortex; vesicle membrane
Molecular Function: dopamine D2 receptor-like receptor activity; identical protein binding; protein binding; potassium channel regulator activity; dopamine receptor activity; dopamine binding; drug binding; SH3 domain binding
Biological Process: circadian rhythm; fear response; synaptic transmission, dopaminergic; behavioral fear response; positive regulation of dopamine uptake; short-term memory; adult locomotory behavior; activation of MAPK activity; positive regulation of kinase activity; regulation of neurotransmitter secretion; regulation of calcium-mediated signaling; behavioral response to ethanol; dopamine metabolic process; dopamine receptor, adenylate cyclase inhibiting pathway; regulation of inhibitory postsynaptic membrane potential; negative regulation of cAMP biosynthetic process; behavioral response to cocaine; negative regulation of protein secretion; negative regulation of adenylate cyclase activity; response to amphetamine; regulation of dopamine metabolic process; social behavior; arachidonic acid secretion; regulation of circadian rhythm; cellular calcium ion homeostasis; olfactory learning; response to steroid hormone stimulus; retina development in camera-type eye; photoperiodism; dopamine receptor signaling pathway; positive regulation of sodium:hydrogen antiporter activity
Disease: Novelty Seeking Personality Trait; Attention Deficit-hyperactivity Disorder
Research Articles on DRD4
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Product Notes
The Rat DRD4 drd4 (Catalog #AAA2503768) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2503768 ELISA Kit recognizes Rat DRD4. It is sometimes possible for the material contained within the vial of "DRD4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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