14-3-3 sigma / Stratifin / YWHAS Recombinant Protein | 14-3-3 sigma recombinant protein

Recombinant Mouse 14-3-3 sigma / Stratifin / YWHAS Protein

Cat. Num. AAA2546904

• Gene Names: Sfn; Er; Mme1; Ywhas
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: 14-3-3 sigma / Stratifin / YWHAS; Recombinant Mouse 14-3-3 sigma / Stratifin / YWHAS Protein; RP23-137L22. 11-001; Er; Mme1; Ywhas; 14-3-3 sigma recombinant protein

• Host: E Coli
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 8.0
• Sequence Length: 248

• Application Notes: The recombinant mouse SFN consists of 250 amino acids and has a calculated molecular mass of 27.7 kDa. It migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gly
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for 14-3-3 sigma recombinant protein

Background: 14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

Description: A DNA sequence encoding the mouse SFN (NP_061224.2) (Met 1-Ser 248) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminu.

NCBI and Uniprot Product Information

• NCBI GI #: 134023662
• NCBI GeneID: 55948
• NCBI Accession #: NP_061224.2
• NCBI GenBank Nucleotide #: NM_018754.2
• UniProt Accession #: O70456; Q3TEZ1
• Molecular Weight: 27,706 Da
• NCBI Official Full Name: 14-3-3 protein sigma
• NCBI Official Synonym Full Names: stratifin
• NCBI Official Symbol: Sfn
• NCBI Official Synonym Symbols: Er; Mme1; Ywhas
• NCBI Protein Information: 14-3-3 protein sigma
• UniProt Protein Name: 14-3-3 protein sigma
• UniProt Gene Name: Sfn
• UniProt Synonym Gene Names: Mkrn3

Uniprot Description

14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4 D2.3|4 66.25 cM

Cellular Component: cell-cell adherens junction; cytoplasm; nucleus

Molecular Function: phosphoprotein binding; protein binding; protein kinase binding

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; keratinization; keratinocyte differentiation; negative regulation of caspase activity; positive regulation of cell growth; positive regulation of epidermal cell differentiation; positive regulation of protein export from nucleus; regulation of cell cycle; regulation of cyclin-dependent protein kinase activity; regulation of epidermal cell division; release of cytochrome c from mitochondria; skin development