Phosphoinositide 3 Kinase, p85 alpha, p110 beta Active Protein | PIK3R1 active protein

Phosphoinositide 3 Kinase, p85 alpha, p110 beta, Recombinant, Human (PI3K)

Cat. Num. AAA635627

• Gene Names: PIK3R1; p85; AGM7; GRB1; p85-ALPHA
• Purity: Highly Purified; 90% (SDS-PAGE)
• Synonyms: Phosphoinositide 3 Kinase; p85 alpha; p110 beta; Recombinant; Human (PI3K); PIK3R1 active protein

• Host: Sf9 insect cells
• Purity/Purification: Highly Purified; 90% (SDS-PAGE)
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 100mM NaCl, 2.5mM MgCl2, and 50% glycerol.

• Biological Activity: 3 nmol/mg/min using phosphatidylinositol as the substrate.
• Preparation and Storage: May be stored at 4 degree C for short-term only For long-term storage, add a desiccate and store at -20 degree C. For long-term storage it is recommended to add a carrier protein, such as 0.1% HSA or BSA. Please avoid freeze-thaw cycles.

Related Product Information for PIK3R1 active protein

The PI3Kb isoform can be activated by insulin via the insulin receptor to initiate a cascade of events that control cell growth and metabolism. The activation of PI3Kb is mediated by the p85 regulatory subunit binding to tyrosine phosphorylated insulin receptor substrate (IRS) proteins (e.g. IRS-1 and IRS-2). It was also shown that PI3Kb is involved in apoptosis in human colon carcinoma cells. Injection of neutralizing antibodies specific to p110b in WiDr, HCT116 and CO 115 adenocarcinoma cells inhibited de novo DNA synthesis. PI3Kb is the major PI3K isoform required for apoptotic cell and Fc-g receptor mediated phagocytosis shown for primary mouse macrophages and the Jurkat human leukemia T cell line. It was shown by several research groups that the catalytic subunit of PI3Kb can be activated by Gbg subunits of G-protein coupled receptors. Recombinant Human Phosphoinositide 3-kinase beta is a glycosilated protein having a molecular weight as follows: p85 chain 83.5kD, p110 chain 124.3kD.

Product Categories/Family for PIK3R1 active protein

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 32455250
• NCBI GeneID: 5295
• NCBI Accession #: NP_852665.1
• NCBI GenBank Nucleotide #: NM_181524.1
• UniProt Accession #: P27986; Q15747; Q4VBZ7; Q53EM6; Q8IXA2; Q8N1C5; B3KT19; D3DWA0; E7EX19
• Molecular Weight: p85 chain 83.5kD, p110 chain 124.3kD
• NCBI Official Full Name: phosphatidylinositol 3-kinase regulatory subunit alpha isoform 3
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• NCBI Official Symbol: PIK3R1
• NCBI Official Synonym Symbols: p85; AGM7; GRB1; p85-ALPHA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
• Protein Family: Phosphatidylinositol 3-kinase regulatory
• UniProt Gene Name: PIK3R1
• UniProt Synonym Gene Names: GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha
• UniProt Entry Name: P85A_HUMAN

NCBI Description

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Uniprot Description

PIK3R1: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Enzyme, regulatory subunit; Kinase, lipid

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; intercellular junction; nucleus; cytosol; phosphoinositide 3-kinase complex

Molecular Function: insulin binding; insulin-like growth factor receptor binding; protein binding; ErbB-3 class receptor binding; neurotrophin TRKA receptor binding; insulin receptor substrate binding; phosphoinositide 3-kinase binding; phosphoinositide 3-kinase regulator activity; protein phosphatase binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; transcription factor binding; insulin receptor binding

Biological Process: negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of RNA splicing; protein amino acid phosphorylation; T cell receptor signaling pathway; NFAT protein import into nucleus; regulation of phosphoinositide 3-kinase activity; positive regulation of glucose import; phosphoinositide phosphorylation; phospholipid metabolic process; positive regulation of transcription factor import into nucleus; epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; protein stabilization; negative regulation of cell-matrix adhesion; positive regulation of tumor necrosis factor production; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; B cell differentiation; T cell costimulation; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration; positive regulation of cell migration; negative regulation of apoptosis

Disease: Agammaglobulinemia 7, Autosomal Recessive; Short Syndrome; Immunodeficiency 36