Phosphoinositide 3 Kinase, Bovine, p110 alpha, p85 alpha Active Protein | PIK3C2B active protein

Phosphoinositide 3 Kinase, Recombinant, Bovine, p110 alpha, p85 alpha (PI3K)

Cat. Num. AAA636613

• Gene Names: PIK3C2B; C2-PI3K
• Purity: Highly Purified; 90% (SDS-PAGE)
• Synonyms: Phosphoinositide 3 Kinase; Bovine; p110 alpha; p85 alpha; Recombinant; p85 alpha (PI3K); PIK3C2B active protein

• Host: Sf9 insect cells
• Purity/Purification: Highly Purified; 90% (SDS-PAGE)
• Form/Format: Supplied as a liquid in 25mM HEPES, pH 8.0, 25mM NaCl, 2.5mM MgCl2 and 50% glycerol.

• Biological Activity: ~ 1 nmol/mg/min using phosphatidylinositol as the substrate.
• Preparation and Storage: Recombinant bovine PI3Ka although stable at 14 degree C for 1 week, should be stored desiccated below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles.

Related Product Information for PIK3C2B active protein

PI3Ka plays a specific role in apoptosis in human colon cancer cells. Injection of neutralizing antibodies specific to PI3Ka into adenocarcinoma cells induced apoptosis, a response that was reverted by treating cells with caspase inhibitor. It was also shown that PI3Ka mediated phosphorylation of the p85a adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3'-phosphorylated phosphoinositides. PI3Ka is a key regulator of the initiation of keratinocyte differentiation. A decrease in PI3Ka activity results in a loss of keratinocyte adhesion to the extracellular membrane and the initiation of early phase differentiation. The PI3Ka catalytic and regulatory subunits are coexpressed in Sf9 insect cells. Recombinant Bovine Phosphoinositide 3-kinase alpha is a glycosylated protein having a molecular weight as follows: p85a chain 83.5kD, p110 chain 124.3kD.

Product Categories/Family for PIK3C2B active protein

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 2076604
• NCBI GeneID: 5287
• UniProt Accession #: O00750; O95666; Q5SW99
• Molecular Weight: p85a chain 83.5kD; p110 chain 124.3kD
• NCBI Official Full Name: phosphoinositide 3-kinase
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta
• NCBI Official Symbol: PIK3C2B
• NCBI Official Synonym Symbols: C2-PI3K
• NCBI Protein Information: phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta; PI3K-C2beta; PI3K-C2-beta; PTDINS-3-kinase C2 beta; ptdIns-3-kinase C2 subunit beta; phosphoinositide 3-kinase-C2-beta; phosphoinositide-3-kinase, class 2, beta polypeptide; phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide; phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta
• Protein Family: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing
• UniProt Gene Name: PIK3C2B
• UniProt Synonym Gene Names: PI3K-C2-beta
• UniProt Entry Name: P3C2B_HUMAN

NCBI Description

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner. [provided by RefSeq, Jul 2008]

Uniprot Description

PIK3C2B: Phosphorylates PtdIns and PtdIns4P with a preference for PtdIns. Does not phosphorylate PtdIns(4,5)P2. May be involved in EGF and PDGF signaling cascades. Belongs to the PI3/PI4-kinase family.

Protein type: Motility/polarity/chemotaxis; Kinase, lipid; EC 2.7.1.154; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: endoplasmic reticulum; endocytic vesicle; plasma membrane; cytosol; nucleus; phosphoinositide 3-kinase complex

Molecular Function: lipid kinase activity; protein binding; 1-phosphatidylinositol-3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide binding; ATP binding

Biological Process: phosphoinositide-mediated signaling; phosphoinositide phosphorylation; phospholipid metabolic process; protein kinase B signaling cascade; phosphatidylinositol biosynthetic process