Glucuronidase Beta Active Protein | GUSB active protein
Recombinant Human Glucuronidase Beta
Gene Names
GUSB; BG; MPS7
Purity
Greater than 90.0% as determined by SDS-PAGE
Synonyms
Glucuronidase Beta; Recombinant Human Glucuronidase Beta; GUSB; BG; MPS7; EC 3.2.1.31; Beta-G1; Beta-D-Glucuronidase; Glucuronidase; Beta; Beta-Glucuronidasem; GUSB Human; Glucuronidase Beta Human Recombinant; GUSB active protein
Host
Sf9, Baculovirus cells
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE
Form/Format
Sterile filtered colorless solution
Protein solution (0.25mg/ml) containing PhosphateBuffered Saline (pH 7.4) and 10% glycerol
Protein solution (0.25mg/ml) containing PhosphateBuffered Saline (pH 7.4) and 10% glycerol
Sequence
LQGGMLYPQESPSRECKELD GLWSFRADFS DNRRRGFEEQ WYRRPLWESG PTVDMPVPSS FNDISQDWRL RHFVGWVWYEREVILPERWT QDLRTRVVLR IGSAHSYAIV WVNGVDTLEH EGGYLPFEAD ISNLVQVGPL PSRLRITIAINNTLTPTTLP PGTIQYLTDT SKYPKGYFVQ NTYFDFFNYA GLQRSVLLYT TPTTYIDDIT VTTSVEQDSGLVNYQISVKG SNLFKLEVRL LDAENKVVAN GTGTQGQLKV PGVSLWWPYL MHERPAYLYS LEVQLTAQTSLGPVSDFYTL PVGIRTVAVT KSQFLINGKP FYFHGVNKHE DADIRGKGFD WPLLVKDFNL LRWLGANAFRTSHYPYAEEV MQMCDRYGIV VIDECPGVGL ALPQFFNNVS LHHHMQVMEE VVRRDKNHPA VVMWSVANEPASHLESAGYY LKMVIAHTKS LDPSRPVTFV SNSNYAADKG APYVDVICLN SYYSWYHDYG HLELIQLQLATQFENWYKKY QKPIIQSEYG AETIAGFHQD PPLMFTEEYQ KSLLEQYHLG LDQKRRKYVV GELIWNFADFMTEQSPTRVL GNKKGIFTRQ RQPKSAAFLL RERYWKIANE TRYPHSVAKS QCLENSLFTH HHHHH.
Sequence Length
651
Biological Activity
Specific activity is > 1600 pmol/min/ug and is definedas the amount of enzyme that hydrolyze 1.0 pmole of 4-Methylumbelliferone to 4-Methylum-belliferyl-beta-D-glucosiduronic acid per minute at 37C and pH6.0
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at-20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles
Related Product Information for GUSB active protein
Description: GUSB Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 635 amino acids (23-651a.a) and having a molecular mass of 73.4kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). GUSB is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Introduction: GlucuronidaseBeta (GUSB) is a lysosomal hydrolase which is taking part in the stepwisedegradation of glucuronic acid-containing glycosaminoglycans and plays a significantrole in the degradation of dermatan and keratin sulfates. GUSB is comprised ofheparin sulfate, chondroitin sulfate and hyaluronan.
Introduction: GlucuronidaseBeta (GUSB) is a lysosomal hydrolase which is taking part in the stepwisedegradation of glucuronic acid-containing glycosaminoglycans and plays a significantrole in the degradation of dermatan and keratin sulfates. GUSB is comprised ofheparin sulfate, chondroitin sulfate and hyaluronan.
Product Categories/Family for GUSB active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
58,345 Da
NCBI Official Full Name
Beta-glucuronidase
NCBI Official Synonym Full Names
glucuronidase beta
NCBI Official Symbol
GUSB
NCBI Official Synonym Symbols
BG; MPS7
NCBI Protein Information
beta-glucuronidase
UniProt Protein Name
Beta-glucuronidase
Protein Family
UniProt Gene Name
GUSB
NCBI Description
This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.[provided by RefSeq, May 2014]
Uniprot Description
Plays an important role in the degradation of dermatan and keratan sulfates.
Research Articles on GUSB
Similar Products
Product Notes
The GUSB gusb (Catalog #AAA147085) is an Active Protein produced from Sf9, Baculovirus cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: LQGGMLYPQE SPSRECKELD GLWSFRADFS DNRRRGFEEQ WYRRPLWESG PTVDMPVPSS FNDISQDWRL RHFVGWVWYE REVILPERWT QDLRTRVVLR IGSAHSYAIV WVNGVDTLEH EGGYLPFEAD ISNLVQVGPL PSRLRITIAI NNTLTPTTLP PGTIQYLTDT SKYPKGYFVQ NTYFDFFNYA GLQRSVLLYT TPTTYIDDIT VTTSVEQDSG LVNYQISVKG SNLFKLEVRL LDAENKVVAN GTGTQGQLKV PGVSLWWPYL MHERPAYLYS LEVQLTAQTS LGPVSDFYTL PVGIRTVAVT KSQFLINGKP FYFHGVNKHE DADIRGKGFD WPLLVKDFNL LRWLGANAFR TSHYPYAEEV MQMCDRYGIV VIDECPGVGL ALPQFFNNVS LHHHMQVMEE VVRRDKNHPA VVMWSVANEP ASHLESAGYY LKMVIAHTKS LDPSRPVTFV SNSNYAADKG APYVDVICLN SYYSWYHDYG HLELIQLQLA TQFENWYKKY QKPIIQSEYG AETIAGFHQD PPLMFTEEYQ KSLLEQYHLG LDQKRRKYVV GELIWNFADF MTEQSPTRVL GNKKGIFTRQ RQPKSAAFLL RERYWKIANE TRYPHSVAKS QCLENSLFTH HHHHH. It is sometimes possible for the material contained within the vial of "Glucuronidase Beta, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
