GUSB active protein

GUSB, 23-651aa, Human, His-tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206501

• Gene Names: GUSB; BG; MPS7
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: GUSB; 23-651aa; Human; His-tag; Baculovirus (Bioactivity Validated); Beta-glucuronidase isoform 1; BG; MPS7; GUSB active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: LQGGMLYPQE SPSRECKELD GLWSFRADFS DNRRRGFEEQ WYRRPLWESG PTVDMPVPSS FNDISQDWRL RHFVGWVWYE REVILPERWT QDLRTRVVLR IGSAHSYAIV WVNGVDTLEH EGGYLPFEAD ISNLVQVGPL PSRLRITIAI NNTLTPTTLP PGTIQYLTDT SKYPKGYFVQ NTYFDFFNYA GLQRSVLLYT TPTTYIDDIT VTTSVEQDSG LVNYQISVKG SNLFKLEVRL LDAENKVVAN GTGTQGQLKV PGVSLWWPYL MHERPAYLYS LEVQLTAQTS LGPVSDFYTL PVGIRTVAVT KSQFLINGKP FYFHGVNKHE DADIRGKGFD WPLLVKDFNL LRWLGANAFR TSHYPYAEEV MQMCDRYGIV VIDECPGVGL ALPQFFNNVS LHHHMQVMEE VVRRDKNHPA VVMWSVANEP ASHLESAGYY LKMVIAHTKS LDPSRPVTFV SNSNYAADKG APYVDVICLN SYYSWYHDYG HLELIQLQLA TQFENWYKKY QKPIIQSEYG AETIAGFHQD PPLMFTEEYQ KSLLEQYHLG LDQKRRKYVV GELIWNFADF MTEQSPTRVL GNKKGIFTRQ RQPKSAAFLL RERYWKIANE TRYPHSVAKS QCLENSLFTH HHHHH
• Sequence Length: 651

• Applicable Applications for GUSB active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 1600 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-Methylumbelliferone to 4-Methylum-belliferyl-beta-D-glucosiduronic acid per minute at 37C and pH6.0
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GUSB active protein

GUSB, also known as beta-glucuronidase isoform 1, is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It includes heparin sulfate, chondroitin sulfate and hyaluronan. Mutations in the GUSB are linked to mucopolysaccharidosis type VII. GUSB plays an important role in the degradation of dermatan and keratin sulfates. Recombinant human GUSB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for GUSB active protein

Signal Transduction; Metabolism

References

Shipley JM., et al. (1993) Am J Hum Genet. 52:517-526.; Bell CE Jr., et al. (1977) J Clin Invest. 59:97-105.

NCBI and Uniprot Product Information

• NCBI GI #: 268834192
• NCBI GeneID: 2990
• NCBI Accession #: NP_000172
• NCBI GenBank Nucleotide #: NM_000181.3
• UniProt Accession #: P08236; Q549U0; Q96CL9; B4E1F6; E9PCV0
• Molecular Weight: 73.4kDa (635aa) 70-100KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: beta-glucuronidase isoform 1
• NCBI Official Synonym Full Names: glucuronidase beta
• NCBI Official Symbol: GUSB
• NCBI Official Synonym Symbols: BG; MPS7
• NCBI Protein Information: beta-glucuronidase
• UniProt Protein Name: Beta-glucuronidase
• Protein Family: Beta-glucuronidase
• UniProt Gene Name: GUSB

NCBI Description

This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.[provided by RefSeq, May 2014]

Uniprot Description

Plays an important role in the degradation of dermatan and keratan sulfates.