Human Coagulation Factor XIII (F13) ELISA Kit | F13 elisa kit

Human Coagulation Factor XIII (F13) ELISA Kit

Cat. Num. AAA2702259

• Gene Names: F13A1; F13A
• Reactivity: Human
• Synonyms: Coagulation Factor XIII (F13); Human Coagulation Factor XIII (F13) ELISA Kit; FXIII; Fibrin Stabilizing Factor; F13 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Coagulation Factor XIII (F13).; No significant cross-reactivity or interference between Coagulation Factor XIII (F13) and analogues was observed.
• Sequence Length: 732

• Assay Type: Double-antibody Sandwich
• Samples: Plasma
• Detection Range: 25-1,600pg/mL
• Sensitivity: < 8pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor XIII (F13) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor XIII (F13) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F13 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor XIII (F13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor XIII (F13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor XIII (F13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor XIII (F13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119395709
• NCBI GeneID: 2162
• NCBI Accession #: NP_000120.2
• NCBI GenBank Nucleotide #: NM_000129.3
• UniProt Accession #: P00488; Q59HA7; Q8N6X2; Q96P24; Q9BX29
• Molecular Weight: 83,267 Da
• NCBI Official Full Name: coagulation factor XIII A chain
• NCBI Official Synonym Full Names: coagulation factor XIII A chain
• NCBI Official Symbol: F13A1
• NCBI Official Synonym Symbols: F13A
• NCBI Protein Information: coagulation factor XIII A chain
• UniProt Protein Name: Coagulation factor XIII A chain
• Protein Family: 36 kDa major membrane protein
• UniProt Gene Name: F13A1
• UniProt Synonym Gene Names: F13A; Coagulation factor XIIIa

NCBI Description

This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]

Uniprot Description

Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.