COX 2, His-tag Active Protein | CPOX active protein

COX 2, His-tag, Recombinant, Human (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)

Cat. Num. AAA635779

• Gene Names: CPOX; CPO; CPX; HCP
• Purity: Purified; 0.7
• Synonyms: COX 2; His-tag; Recombinant; Human (Cyclooxygenase 2; PGHS 2; Prostaglandin Endoperoxide Synthase 2; PHS 2); CPOX active protein

• Host: Sf21 cells.
• Purity/Purification: Purified; 0.7
• Form/Format: Supplied as a liquid in 80mM Tris-HCl, pH 8.0, 0.1% Tween 20, 300uM DDC.

• Specific Activity: 8000 units/mg protein.
• Unit Definition: One unit of enzyme consumes one nmol of oxygen per minute at 37 degree C in 0.1M Tris-HCl buffer, pH 8.0, 100uM arachidonate, 5mM EDTA, 2mM phenol, and 1uM hematin.
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.. The enzyme preparation contains diethyldithiocarbamate (DDC) as a preservative. If this preservative is undesirable, it can be removed by standard desalting procedures. However, the enzyme is extremely unstable in the absence of the preservative and should be used within one hour.

Related Product Information for CPOX active protein

The prostanoid family includes PGD2, PGE2, PGF2alpha, PGI2, thromboxane A2 and prostaglandins. The prostaglandins (PGs) are implicated in various physiological and pathophysiological events, including male fertility, menstruation, ovulation, pregnancy, implantation and inflamatory and neoplastic diseases. The biosynthesis of PGs and some other prostanoids, is catalyzed in a rate limiting step by PG-H synthase (also known as cyclooxygenase (COX), PG-endoperoxidase synthase (PTGS)) which converts arachidonic acid to prostaglandin/prostanoid precursor PGH2.

Two cyclooxygenase isozymes, COX1 (human, 576aa, 69-72kD; chromosome 9) and COX2 (human, 604aa, 74kD; chromosome 1) have been identified. COX1, a constitutively expressed isoform, produces physiologically relevant prostanoids such as those in stomach and platelets. COX2 isoform is inducible and rapidly upregulated at inflamation sites and forms proinflamatory prostanoids. The overexpression of COX-2 also leads to tumerogenesis.
Recently, a third isoform COX3 (canine 633aa; ~65kD in human aorta) has been reported. Two smaller COX1-derived proteins (partial COX1) PCOX1a (canine 414aa, ~53kD in human aorta) and PCOX1b have also been characterized. The COX3, but not PCOX1a, possesses glycosylation dependent cyclooxygenase activity.
The nonsteroidal antiinflammatory drugs (NSAIDs) reduce the formation of prostaglandins by inhibiting the activity of cyclooxygenases (COX1, COX2 and COX3), This ability was associated with inhibition of COX, which converts arachidonic acid to the prostaglandin precursor prostaglandin H2.

Product Categories/Family for CPOX active protein

Molecular Biology; MB-Enzymes; Cyclooxygenase (COX)

NCBI and Uniprot Product Information

• NCBI GI #: 41393599
• NCBI GeneID: 1371
• NCBI Accession #: NP_000088.3
• NCBI GenBank Nucleotide #: NM_000097.5
• UniProt Accession #: P36551; Q14060; Q53F08; Q8IZ45; Q96AF3; A8K275
• Molecular Weight: 50,152 Da
• NCBI Official Full Name: coproporphyrinogen-III oxidase, mitochondrial
• NCBI Official Synonym Full Names: coproporphyrinogen oxidase
• NCBI Official Symbol: CPOX
• NCBI Official Synonym Symbols: CPO; CPX; HCP
• NCBI Protein Information: coproporphyrinogen-III oxidase, mitochondrial; COX; coprogen oxidase; coproporphyrinogenase
• UniProt Protein Name: Coproporphyrinogen-III oxidase, mitochondrial
• Protein Family: Oxygen-dependent coproporphyrinogen-III oxidase
• UniProt Gene Name: CPOX
• UniProt Synonym Gene Names: CPO; CPX; COX; Coprogen oxidase
• UniProt Entry Name: HEM6_HUMAN

NCBI Description

The protein encoded by this gene is the sixth enzyme of the heme biosynthetic pathway. The encoded enzyme is soluble and found in the intermembrane space of mitochondria. This enzyme catalyzes the stepwise oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX, a precursor of heme. Defects in this gene are a cause of hereditary coproporphyria (HCP).[provided by RefSeq, Oct 2009]

Uniprot Description

CPOX: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III. Defects in CPOX are the cause of hereditary coproporphyria (HCP). HCP is an acute hepatic porphyria and an autosomal dominant disease characterized by neuropsychiatric disturbances and skin photosensitivity. Biochemically, there is an overexcretion of coproporphyrin III in the urine and in the feces. HCP is clinically characterized by attacks of abdominal pain, neurological disturbances, and psychiatric symptoms. The symptoms are generally manifested with rapid onset, and can be precipitated by drugs, alcohol, caloric deprivation, infection, endocrine factors or stress. A severe variant form is harderoporphyria, which is characterized by earlier onset attacks, massive excretion of harderoporphyrin in the feces, and a marked decrease of coproporphyrinogen IX oxidase activity. Belongs to the aerobic coproporphyrinogen-III oxidase family.

Protein type: Mitochondrial; EC 1.3.3.3; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Oxidoreductase

Chromosomal Location of Human Ortholog: 3q12

Cellular Component: mitochondrion; mitochondrial inner membrane; cytoplasm; mitochondrial intermembrane space

Molecular Function: coproporphyrinogen oxidase activity; protein homodimerization activity; structural constituent of eye lens

Biological Process: response to arsenic; response to methylmercury; response to lead ion; porphyrin metabolic process; response to insecticide; protoporphyrinogen IX biosynthetic process; response to iron ion; heme biosynthetic process

Disease: Coproporphyria, Hereditary