PR-Set7 Recombinant Protein | KMT5A recombinant protein

PR-Set7 Protein, Human, Recombinant

Cat. Num. AAA8120952

• Gene Names: SETD8; SET8; KMT5A; SET07; PR-Set7
• Purity: >98% as determined by SDS-PAGE
• Synonyms: PR-Set7; PR-Set7 Protein; Human; Recombinant; Human SETD8/PR-Set7 Protein; KMT5A Protein; SET07 Protein; SET8 Protein; SET domain containing (lysine methyltransferase) 8; KMT5A recombinant protein

• Host: E Coli
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 100mM NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Lys195-His352

• Species: Human
• Predicted N Terminal: Gly
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the human SETD8 (NP_065115.3)(Lys 195-His 352) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for KMT5A recombinant protein

Background: KMT5A (known as PR-Set7/9, SETD8 and SET8), a member of the SET domain containing methyltransferase family specifically targeting H4K20 for methylation, has been implicated in multiple biological processes. Inhibition of KMT5A attenuated proliferation and induced apoptosis. Elevated KMT5A expression was significantly correlated with extrathyroidal extension, lymph node metastasis and advanced pathological stage of papillary thyroid cancer. KMT5A may be a novel oncogenic factor, specifically a regulator for lipid metabolism in papillary thyroid carcinoma.

NCBI and Uniprot Product Information

• NCBI GI #: 45356743
• NCBI GeneID: 387893
• NCBI Accession #: NP_065115.3
• NCBI GenBank Nucleotide #: NM_020382.3
• UniProt Accession #: Q9NQR1; Q86W83; Q8TD09; A8K9D0
• Molecular Weight: 39,223 Da
• NCBI Official Full Name: N-lysine methyltransferase SETD8
• NCBI Official Synonym Full Names: SET domain containing (lysine methyltransferase) 8
• NCBI Official Symbol: SETD8
• NCBI Official Synonym Symbols: SET8; KMT5A; SET07; PR-Set7
• NCBI Protein Information: N-lysine methyltransferase SETD8; PR/SET07; H4-K20-HMTase SETD8; lysine N-methyltransferase 5A; SET domain-containing protein 8; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; histone-lysine N-methyltransferase SETD8; H4-K20-spec
• UniProt Protein Name: N-lysine methyltransferase SETD8
• Protein Family: N-lysine methyltransferase
• UniProt Gene Name: SETD8
• UniProt Synonym Gene Names: KMT5A; PRSET7; SET07; SET8; PR-Set7; PR/SET07
• UniProt Entry Name: SETD8_HUMAN

Uniprot Description

SETD8: a protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Methylates K20 of histone H4 (H4K20me1), a specific tag for epigenetic transcriptional repression. SETD8 protein and H4K20me1 levels are cell cycle regulated, both increasing in S phase and peaking at G2/M phase. Interacts with the PCNA protein, associates with sites of active DNA synthesis and is required for DNA replication and genome stability during S phase. Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Nucleosomes are preferred as substrate compared to free histones. Methylates p53K382, leading to repression of the pro-apoptotic and checkpoint activation functions of p53. SET8 expression levels decrease in response to DNA damage, allowing p53 to activate checkpoints and/or apoptosis. Both the methylation of histone H4K20 and p53K382 appear to be important for the functions of SET8 in S phase. Belongs to the histone-lysine methyltransferase family. PR/SET subfamily. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase, protein lysine; Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; nucleolus; chromosome; nucleus

Molecular Function: protein binding; p53 binding; protein-lysine N-methyltransferase activity; transcription corepressor activity; histone-lysine N-methyltransferase activity

Biological Process: mitosis; transcription, DNA-dependent; cell division; peptidyl-lysine mono-methylation; negative regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; regulation of DNA damage response, signal transduction by p53 class mediator