SDS-Page
(Recombinant SETD3 protein gel 10% SDS-PAGE Coomassie staining MW: 68.5 kDa Purity: >90%)
SETD3 recombinant protein
Recombinant SETD3 protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant SETD3 protein gel 10% SDS-PAGE Coomassie staining MW: 68.5 kDa Purity: >90%)
SDS-Page
(Recombinant SETD3 protein gel 10% SDS-PAGE Coomassie staining MW: 68.5 kDa Purity: >90%)
Testing Data
(MTase-Glo Assay for Recombinant SETD3 protein methyltransferase activity 1 uM beta-actin peptide (66-TLKYPIEHGIVTNWD-80) and 1 uM SAM were incubated with different concentrations of recombinant SETD3 protein in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol.)
Testing Data
(MTase-Glo Assay for Recombinant SETD3 protein methyltransferase activity 1 uM beta-actin peptide (66-TLKYPIEHGIVTNWD-80) and 1 uM SAM were incubated with different concentrations of recombinant SETD3 protein in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol.)
Testing Data
(MTase-Glo Assay for Recombinant SETD3 protein methyltransferase activity 1 uM beta-actin peptide (66-TLKYPIEHGIVTNWD-80) and 1 uM SAM were incubated with different concentrations of recombinant SETD3 protein in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol.)
Testing Data
(MTase-Glo Assay for Recombinant SETD3 protein methyltransferase activity 1 uM beta-actin peptide (66-TLKYPIEHGIVTNWD-80) and 1 uM SAM were incubated with different concentrations of recombinant SETD3 protein in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol.)
Background: SETD3 (SET Domain Containing 3, Actin Histidine Methyltransferase), is a protein-histidine N-methyltransferase that specifically mediates methylation of actin at 'His-73'. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery. According to researches, SETD3 does not have proteinlysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin.
NCBI and Uniprot Product Information
Uniprot Description
SETD3: Histone methyltransferase that methylates 'Lys-36' of histone H3 (H3K36me). H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Belongs to the SETD3 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Methyltransferase; EC 2.1.1.43; Methyltransferase, protein lysine
Chromosomal Location of Human Ortholog: 14q32.2
Cellular Component: nuclear chromatin
Molecular Function: histone lysine N-methyltransferase activity (H3-K4 specific); transcription coactivator activity; histone lysine N-methyltransferase activity (H3-K36 specific)
Biological Process: transcription, DNA-dependent; peptidyl-lysine di-methylation; positive regulation of transcription, DNA-dependent; peptidyl-lysine tri-methylation; histone H3-K4 methylation; peptidyl-lysine mono-methylation; positive regulation of muscle cell differentiation; histone H3-K36 methylation; positive regulation of transcription from RNA polymerase II promoter
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Product Notes
The SETD3 setd3 (Catalog #AAA389328) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SETD3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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