Rat Protein Kinase C ELISA Kit | PKC elisa kit

Rat Protein Kinase C ELISA Kit

Cat. Num. AAA763422

• Gene Names: PKC1; CLY15; CLY5; CLY7; HPO2; STT1
• Reactivity: Rat
• Synonyms: Protein Kinase C; Rat Protein Kinase C ELISA Kit; PKC; PKC elisa kit

• Reactivity: Rat
• Sequence Length: 1151

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PKC elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat PKC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat PKC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat PKC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat PKC can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 330443395
• NCBI GeneID: 852169
• NCBI Accession #: NP_009445.2
• NCBI GenBank Nucleotide #: NM_001178345.2
• Molecular Weight: 131,477 Da
• NCBI Official Full Name: protein kinase C
• NCBI Official Symbol: PKC1
• NCBI Official Synonym Symbols: CLY15; CLY5; CLY7; HPO2; STT1
• NCBI Protein Information: protein kinase C
• UniProt Protein Name: Protein kinase C-like 1
• Protein Family: Protein kinase
• UniProt Gene Name: PKC1
• UniProt Synonym Gene Names: HPO2; STT1; PKC 1
• UniProt Entry Name: KPC1_YEAST

Uniprot Description

Required for cell growth and for the G2->M transition of the cell division cycle. Mediates a protein kinase cascade; it activates BCK1 which itself activates MKK1/MKK2.