PEDF recombinant protein

Human PEDF Recombinant

Cat. Num. AAA553066

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: PEDF; Human PEDF Recombinant; pedf; serpinf1; PEDF recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant PEDF was lyophilized from a 0.2 um filtered 20mM PB,150mM NaCl solution pH 7.5.
• Sequence: FGYDLYRVRS STSPTTNVLL SPLSVATALS ALSLGAEQRT ESIIHRALYY DLISSPDIHG TYKELLDTVT APQKNLKSAS RIVFEKKLRI KSSFVAPLEK SYGTRPRVLT GNPRLDLQEI NNWVQAQMKG KLARSTKEIP DEISILLLGV AHFKGQWVTK FDSRKTSLED FYLDEERTVR VPMMSDPKAV LRYGLDSDLS CKIAQLPLTG SMSIIFFLPL KVTQNLTLIE ESLTSEFIHD IDRELKTVQA VLTVPKLKLS YEGEVTKSLQ EMKLQSLFDS PDFSKITGKP IKLTQVEHRA GFEWNEDGAG TTPSPGLQPA HLTFPLDYHL NQPFIFVLRD TDTGALLFIG KILDPRGP
• Sequence Length: 418

• Domain: SERPIN
• Host Note: Optimized DNA sequence encoding Human PEDF mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human PEDF, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 45kDa.; ; Recombinant PEDF is a disulfide-linked monomer protein consisting of 440 amino acid residue subunits, and migrates as an approximately 45kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for PEDF recombinant protein

Pigment epithelium-derived factor (PEDF), a 50-kDa secreted glycoprotein, is widely expressed throughout the human body, with expression decreasing during human hepatocellular carcinoma and breast cancer progression.PEDF binds to a cell membrane receptor to exhibit multifunctional activity in many cell types but its signaling mechanisms are largely unknown. PEDF exerts anti-angiogenic activity by arresting VEGF- or bFGF-mediated endothelial cell migration, inhibiting capillary morphogenesis, and inducing endothelial cell apoptosis.In human dermal microvascular cells, PEDF induces FasL expression and subsequently activation of caspase-8, which initiates the downstream apoptotic cascade. In human umbilical vein endothelial cells (HUVECs), its induction of apoptosis was shown to depend on p38 MAPK activity and to involve activation of caspases-8 and-9.

Product Categories/Family for PEDF recombinant protein

Cytokines

References

• neuroprotectin d1 synthesis and corneal nerve regeneration after experimental surgery and treatment with PEDF plus dha .Invest. Ophthalmol. Vis. Sci., Feb 2010; 51: 804 - 810.
• anti-permeability function of PEDF involves blockade of map kinase/gsk/beta-catenin signaling pathway and upar expression .Invest. Ophthalmol. Vis. Sci., Jan 2010; 10.1167/iovs.08-2878.
• inhibition of jak2/stat3-mediated vegf upregulation under high glucose conditions by PEDF through a mitochondrial ros pathway in vitro .Invest. Ophthalmol. Vis. Sci., Jan 2010; 51: 64 - 71.
• constitutive overexpression of pigment epithelium–derived factor inhibition of ocular melanoma growth and metastasis .Invest. Ophthalmol. Vis. Sci., Jan 2010; 51: 28 - 34.
• pigment epithelium–derived factor (PEDF) attenuated capsaicin-induced neurotrophic keratouveitis .Invest. Ophthalmol. Vis. Sci., Nov 2009; 50: 5173 - 5180.