Typical Testing Data/Standard Curve (for reference only)
Human serpin peptidase inhibitor, clade D (heparin cofactor) , member 1 ELISA Kit | SERPIND1 elisa kit
Human heparin cofactor II, HCII ELISA Kit
Gene Names
SERPIND1; HC2; LS2; HCF2; HCII; HLS2; THPH10; D22S673
Reactivity
Human
Synonyms
serpin peptidase inhibitor; clade D (heparin cofactor); member 1; Human heparin cofactor II; HCII ELISA Kit; HCIIELISA Kit; D22S673; HC2; HCF2; HCII; HLS2; LS2; OTTHUMP00000198451; heparin cofactor II; leuserpin 2; serine (or cysteine) proteinase inhibitor; SERPIND1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human HC II. No significant cross-reactivity or interference between human HC II and analogues was observed.
Sequence Length
499
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
1.25 pg/ml -80 pg/ml
Sensitivity
0.312 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SERPIND1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HCII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HCII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HCII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCII bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,071 Da
NCBI Official Full Name
heparin cofactor 2
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade D (heparin cofactor), member 1
NCBI Official Symbol
SERPIND1
NCBI Official Synonym Symbols
HC2; LS2; HCF2; HCII; HLS2; THPH10; D22S673
NCBI Protein Information
heparin cofactor 2; serpin D1; leuserpin 2; protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1
UniProt Protein Name
Heparin cofactor 2
Protein Family
UniProt Gene Name
SERPIND1
UniProt Synonym Gene Names
HCF2; HC-II; HLS2
UniProt Entry Name
HEP2_HUMAN
NCBI Description
The product encoded by this gene is a serine proteinase inhibitor which rapidly inhibits thrombin in the presence of dermatan sulfate or heparin. The gene contains five exons and four introns. This protein shares homology with antithrombin III and other members of the alpha 1-antitrypsin superfamily. Mutations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.1 PublicationManual assertion based on experiment in:Ref.11
Research Articles on SERPIND1
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Product Notes
The Human SERPIND1 serpind1 (Catalog #AAA703038) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703038 ELISA Kit recognizes Human SERPIND1. It is sometimes possible for the material contained within the vial of "serpin peptidase inhibitor, clade D (heparin cofactor) , member 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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