Human HS-IFN-gamma (High sensitive Interferon gamma) ELISA Kit | IFN-gamma elisa kit

Human HS-IFN-gamma (High sensitive Interferon gamma) Accquant ELISA Kit

Cat. Num. AAA7612122

• Reactivity: Human
• Synonyms: HS-IFN-gamma (High sensitive Interferon gamma); Human HS-IFN-gamma (High sensitive Interferon gamma) Accquant ELISA Kit; IFN-gamma; IFNG; IFG; IFI; Type II Interferon; IFN-gamma elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IFN-gamma. No significant cross-reactivity or interference between IFN-gamma and analogues were observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 2.34-150pg/ml
• Sensitivity: 1.41pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFN-gamma elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin (HRP-SA) and Biotin Systerm (BS) was added and unbound conjugates were washed away with wash buffer. Then by twice signal amplification, TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

Background/Introduction: Interferon gamma (IFN-gamma) is a cytokine critical to both innate and adaptive immunity, and functions as the primary activator of macrophages, in addition to stimulating natural killer cells and neutrophils. Interferon-gamma (IFN-gamma), also known as type II or immune interferon exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interacts with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up?regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.