Testing Data
()
BseDI Restriction Enzyme
BseDI (SecI)
Purity
Molecular Biology Grade
Synonyms
BseDI; BseDI (SecI); BseDI restriction enzyme
Host
BaCillus stearothermophilus RFL 1434
Purity/Purification
Molecular Biology Grade
Form/Format
R 7625: Restriction Enzyme Buffer A, lOX. Suitable for use in Double Digest assays.
Concentration
10u/ul (varies by lot)
Sequence
5'-C'^C N N G G-3'3'-G G N N C^C-5'
Supplied With
R1625: Restriction Enzyme Buffer A, 10X. Suitable for use in Double Digest assays.
Quality Control
Overdigestion Assay:
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (1 Ou/ug lambda DNA x 16 hours) with BseDI.
Ligation/Recutting Assay: After 50-fold overdigestlon (3u/ug DNA x The Ligation/Recutting assay was replaced with LO test after validating experiements showed LO test ability to trace nuclease and phophatase actiVities with sensitivity that IS higher than L/R by a factor of 10.
Labeled Oligonucleotide (LO) Assay: No detectaBle degradation of a single-stranded and double-stranded labeled oligoilucleotide was obselved after incubation with 10 units of BseDI for 4 hours.
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (1 Ou/ug lambda DNA x 16 hours) with BseDI.
Ligation/Recutting Assay: After 50-fold overdigestlon (3u/ug DNA x The Ligation/Recutting assay was replaced with LO test after validating experiements showed LO test ability to trace nuclease and phophatase actiVities with sensitivity that IS higher than L/R by a factor of 10.
Labeled Oligonucleotide (LO) Assay: No detectaBle degradation of a single-stranded and double-stranded labeled oligoilucleotide was obselved after incubation with 10 units of BseDI for 4 hours.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 55 degree C in 50ul of assay buffer.
Dilution Buffer
10mM Tris-HCl, pH 7.4 at RT, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
Storage Buffer
Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
Direction for use
Incubate at 55 degree C. (Incubation at 37 degree C results in 10% activity.)
Enzyme Properties
Thermal Inactivation:
BseDI is inactivated by incubation at 80 degree C for 20min.
Stability during Prolonged Incubation:
A minimum of 0.2 units of BseDI is required for complete digestion of 1 ug of lambda DNA in 16 hours at 55 degree C.
Number of Recognition Sites in DNA:
Lambda: 105
PhiX174: 6
M13mp18/19: 9
pBR322: 8
pUC18/19: 5
pUC57: 4
pTZ19R/U: 5
Methylation Effects:
DAM: Never overlaps - no effect
Dcm: May overlap - no effect
CpG: May overlap - no effect
EcoKI: Never overlaps - no effect
EcoBI: Never overlaps - no effect
BseDI is inactivated by incubation at 80 degree C for 20min.
Stability during Prolonged Incubation:
A minimum of 0.2 units of BseDI is required for complete digestion of 1 ug of lambda DNA in 16 hours at 55 degree C.
Number of Recognition Sites in DNA:
Lambda: 105
PhiX174: 6
M13mp18/19: 9
pBR322: 8
pUC18/19: 5
pUC57: 4
pTZ19R/U: 5
Methylation Effects:
DAM: Never overlaps - no effect
Dcm: May overlap - no effect
CpG: May overlap - no effect
EcoKI: Never overlaps - no effect
EcoBI: Never overlaps - no effect
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
()
Testing Data
()
Product Categories/Family for BseDI restriction enzyme
Similar Products
Product Notes
The BseDI (Catalog #AAA638488) is a Restriction Enzyme produced from BaCillus stearothermophilus RFL 1434 and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: 5'-C'^C N N G G-3'3'-G G N N C^C-5'. It is sometimes possible for the material contained within the vial of "BseDI, Restriction Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
