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SDS-Page

Thyroxine Binding Globulin (TBG) Recombinant Protein | TBG recombinant protein

Recombinant Thyroxine Binding Globulin (TBG)

Reactivity
Mus musculus (Mouse)
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
Thyroxine Binding Globulin (TBG); Recombinant Thyroxine Binding Globulin (TBG); TBG recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Reactivity
Mus musculus (Mouse)
Purity/Purification
> 97%
Form/Format
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 50ug/mL (varies by lot)
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
NELE LQMGNAVFIG QQLKPLAKFL DDVKTLYETE VFSTDFSNVS AAQHKINSYV EKQTKGKIVG LIQGLKLNII MILVNYIHFR AQWANPFRVS KTEESSNFSV DKSTTVQVPM MHQLEQYYHY VDMELNCTVL QMDYSENALA LFVLPKEGHM EWVEAAMSSK TLKKWNYLLQ KGWVELFVPK FSISATYDLG STLQKMGMRD AFAESADFPG ITEDSGLKLS YAFHKAVLHIGEEGTKEGAS PEVGSLDQQE VPPLHPVIRL DRA
Applicable Applications for TBG recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Asn127~Ala393
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
5.9
Usage
Reconstitute in ddH2O to a concentration of 0.1-0.35 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 31.4kDa
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
Thyroxine-binding globulin
UniProt Protein Name
Thyroxine-binding globulin
UniProt Gene Name
Serpina7
UniProt Synonym Gene Names
Tbg
UniProt Entry Name
THBG_MOUSE

Uniprot Description

SERPINA7: Major thyroid hormone transport protein in serum. Defects in SERPINA7 are a cause of thyroxine-binding globulin deficiency (TBG deficiency). Mutations in the SERPINA7 gene can result as a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. Patients show, respectively, reduced or elevated protein- bound iodine but are euthyroid. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; hormone binding

Biological Process: response to drug; response to vitamin A; post-embryonic development

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Product Notes

The TBG serpina7 (Catalog #AAA2009645) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Thyroxine Binding Globulin (TBG) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Thyroxine Binding Globulin (TBG) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the TBG serpina7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. NELE LQMGNAVFIG QQLKPLAKFL DDVKTLYETE VFSTDFSNVS AAQHKINSYV EKQTKGKIVG LIQGLKLNII MILVNYIHFR AQWANPFRVS KTEESSNFSV DKSTTVQVPM MHQLEQYYHY VDMELNCTVL QMDYSENALA LFVLPKEGHM EWVEAAMSSK TLKKWNYLLQ KGWVELFVPK FSISATYDLG STLQKMGMRD AFAESADFPG ITEDSGLKLS YAFHKAVLHI GEEGTKEGAS PEVGSLDQQE VPPLHPVIRL DRA. It is sometimes possible for the material contained within the vial of "Thyroxine Binding Globulin (TBG), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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